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  Crystal Structures of [Fe]-Hydrogenase from Methanolacinia paynteri Suggest a Path of the FeGP-Cofactor Incorporation Process

Huang, G., Arriaza-Gallardo, F. J., Wagner, T., & Shima, S. (2020). Crystal Structures of [Fe]-Hydrogenase from Methanolacinia paynteri Suggest a Path of the FeGP-Cofactor Incorporation Process. Inorganics, 8(9): 50. doi:10.3390/inorganics8090050.

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Huang, Gangfeng, Author
Arriaza-Gallardo, Francisco Javier, Author
Wagner, Tristan1, Author           
Shima, Seigo, Author
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1Research Group Microbial Metabolism, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_3282402              

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 Abstract: [Fe]-hydrogenase (Hmd) catalyzes the reversible heterolytic cleavage of H-2, and hydride transfer to methenyl-tetrahydromethanopterin (methenyl-H4MPT+). The iron-guanylylpyridinol (FeGP) cofactor, the prosthetic group of Hmd, can be extracted from the holoenzyme and inserted back into the protein. Here, we report the crystal structure of an asymmetric homodimer of Hmd fromMethanolacinia paynteri(pHmd), which was composed of one monomer in the open conformation with the FeGP cofactor (holo-form) and a second monomer in the closed conformation without the cofactor (apo-form). In addition, we report the symmetric pHmd-homodimer structure in complex with guanosine monophosphate (GMP) or guanylylpyridinol (GP), in which each ligand was bound to the protein, where the GMP moiety of the FeGP-cofactor is bound in the holo-form. Binding of GMP and GP modified the local protein structure but did not induce the open conformation. The amino-group of the Lys150 appears to interact with the 2-hydroxy group of pyridinol ring in the pHmd-GP complex, which is not the case in the structure of the pHmd-FeGP complex. Lys150Ala mutation decreased the reconstitution rate of the active enzyme with the FeGP cofactor at the physiological pH. These results suggest that Lys150 might be involved in the FeGP-cofactor incorporation into the Hmd protein in vivo.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000578876600001
DOI: 10.3390/inorganics8090050
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Title: Inorganics
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI
Pages: - Volume / Issue: 8 (9) Sequence Number: 50 Start / End Page: - Identifier: ISSN: 2304-6740
CoNE: https://pure.mpg.de/cone/journals/resource/2304-6740