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  The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and Their Translocation across the Blood-Tissue Border

Uhl, B., Hirn, S., Immler, R., Mildner, K., Möckl, L., Sperandio, M., et al. (2017). The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and Their Translocation across the Blood-Tissue Border. ACS Nano, 11(2), 1498-1508. doi:10.1021/acsnano.6b06812.

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 Creators:
Uhl, Bernd1, Author
Hirn, Stephanie1, Author
Immler, Roland1, Author
Mildner, Karina1, Author
Möckl, Leonhard2, 3, Author           
Sperandio, Markus1, Author
Braeuchle, Christoph1, Author
Reichel, Christoph A.1, Author
Zeuschner, Dagmar1, Author
Krombach, Fritz1, Author
Affiliations:
1external, ou_persistent22              
2External Organizations, ou_persistent22              
3Ludwig-Maximilians-Universität München, ou_persistent22              

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Free keywords: nanoparticles; endothelial glycocalyx; nanoparticle-endothelium interactions; translocation; multiphoton in vivo microscopy; quantum dots; blood-tissue border
 Abstract: Advances in the engineering of nanoparticles (NPs), which represent particles of less than 100 nm in one external dimension, led to an increasing utilization of nanomaterials for biomedical purposes. A prerequisite for their use in diagnostic and therapeutic applications, however, is the targeted delivery to the site of injury. Interactions between blood-borne NPs and the vascular endothelium represent a critical step for nanoparticle delivery into diseased tissue. Here, we show that the endothelial glycocalyx, which constitutes a glycoprotein polysaccharide meshwork coating the luminal surface of vessels, effectively controls interactions of carboxyl-functionalized quantum dots with the micro vascular endothelium. Glycosaminoglycans of the endothelial glycocalyx were found to physically cover endothelial adhesion and signaling molecules, thereby preventing endothelial attachment, uptake, and translocation of these nanoparticles through different layers of the vessel wall. Conversely, degradation of the endothelial glycocalyx promoted interactions of these nanoparticles with microvascular endothelial cells under the pathologic condition of ischemia-reperfusion, thus identifying the injured endothelial glycocalyx as an essential element of the blood-tissue border facilitating the targeted delivery of nanomaterials to diseased tissue.

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Language(s): eng - English
 Dates: 2017-02
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000395357300040
DOI: 10.1021/acsnano.6b06812
 Degree: -

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Title: ACS Nano
Source Genre: Journal
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Publ. Info: American Chemical Society
Pages: - Volume / Issue: 11 (2) Sequence Number: - Start / End Page: 1498 - 1508 Identifier: ISSN: 1936-0851