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  Kinetics of pump currents generated by the Na+,K+-ATPase

Fendler, K., Grell, E., & Bamberg, E. (1987). Kinetics of pump currents generated by the Na+,K+-ATPase. FEBS Letters, 224(1), 83-88. doi:10.1016/0014-5793(87)80427-1.

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 Creators:
Fendler, Klaus1, Author           
Grell, Ernst2, Author           
Bamberg, Ernst1, Author           
Affiliations:
1Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
2Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3264820              

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Free keywords: Na+,K+-ATPase; Black lipid membrane; Transient kinetics; Electrogenic property; Pump current
 Abstract: Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s-1 (22 degrees C). The exponential components found in the transient currents are compared to transitions in the Albers-Post scheme.

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Language(s): eng - English
 Dates: 1987-08-172001-10-191987-11-16
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-5793(87)80427-1
PMID: 2824243
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 224 (1) Sequence Number: - Start / End Page: 83 - 88 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501