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  Immunological characterization and localization of the Na+/Ca2+- exchanger in bovine retina

Haase, W., Friese, W., Gordon, R. D., Müller, H., & Cook, N. J. (1990). Immunological characterization and localization of the Na+/Ca2+- exchanger in bovine retina. The Journal of Neuroscience, 10(5), 1486-1494. doi:10.1523/JNEUROSCI.10-05-01486.1990.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-692E-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-692F-0
Genre: Journal Article

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 Creators:
Haase, Winfried1, Author              
Friese, Waltraud1, Author              
Gordon, Robert D.2, Author              
Müller, Heidi2, Author              
Cook, Neil J.2, Author              
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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 Abstract: The sodium/calcium exchanger was purified from bovine retinal rod outer segment membranes and used for the immunization of New Zealand White rabbits. A polyclonal antibody was produced which was found to bind specifically to the 230 kDa Na+/Ca2+-exchanger protein as assessed by Western blotting. The antibody did not bind to the high-molecular- weight “rim protein,” thereby demonstrating that this protein is distinct from the rod outer segment of Na+/Ca2+-exchanger. We used the polyclonal antibody for immunohistochemically localizing the exchange protein in bovine retina. Fluorescent light microscopy revealed intensive immunolabeling of the photoreceptor outer segments, whereas other retinal cell layers exhibited minimal binding. Using the electron microscopic immunogold method, we found specific antibody binding to the extracellular side of rod outer segment plasma membrane. Rod disk membranes, rod inner segments, and cone photoreceptors displayed no significant labeling. We therefore conclude that the Na+/Ca2+-exchanger is localized primarily in the rod outer segment plasma membrane, the most appropriate localization considering its proposed role in the process of vertebrate phototransduction.

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Language(s): eng - English
 Dates: 1989-11-081989-05-301989-11-241990-05-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1523/JNEUROSCI.10-05-01486.1990
PMID: 2185348
 Degree: -

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Title: The Journal of Neuroscience
  Other : The Journal of Neuroscience: the Official Journal of the Society for Neuroscience
  Abbreviation : J. Neurosci.
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, DC : Society of Neuroscience
Pages: - Volume / Issue: 10 (5) Sequence Number: - Start / End Page: 1486 - 1494 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1