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  Validated MAGIC and GWAS populations mapping reveal the link between vitamin E contents and natural variation in chorismate metabolism in tomato

Burgos, E., Luca, D., Belen, M., Diouf, I., de Haro, A., L., et al. (2020). Validated MAGIC and GWAS populations mapping reveal the link between vitamin E contents and natural variation in chorismate metabolism in tomato. The Plant Journal. Retrieved from https://doi.org/10.1111/tpj.15077.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-862B-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-862C-1
Genre: Journal Article
Alternative Title : The Plant Journal

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 Creators:
Burgos, Estanislao1, Author
Luca, De1, Author
Belen, Maria1, Author
Diouf, Isidore1, Author
de Haro1, Author
A., Luis1, Author
Albert, Elise1, Author
Sauvage, Christopher1, Author
Jian Tao, Zhao1, Author
Bermudez, Luisa1, Author
Asís, Ramon1, Author
Nunes Nesi, Adriano1, Author
Matringe, Michel1, Author
Brehelin, Claire1, Author
Guiraud, Thomas1, Author
Ferrand, Carine1, Author
Atienza, Isabelle1, Author
Jorly, Joana1, Author
Mauxion, Jean Philipppe1, Author
Baldet, Pierre1, Author
Fernie, A. R.2, Author              Quadrana, Leandro1, AuthorRothan, Christophe1, AuthorCausse, Mathilde1, AuthorCarrari, Fernando1, Author more..
Affiliations:
1external, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: Tocopherol, Tocotrienol, Vitamin E, Tomato Fruit, Solanum lycopersicum
 Abstract: Summary Tocochromanols constitute the different forms of vitamin E (VTE), essential for the human diet and display a high membrane protectant activity. By combining interval mapping and genome-wide association studies (GWAS) we unveiled the genetic determinants of tocochromanol accumulation in tomato fruits. To enhance the nutritional value of this highly consumed vegetable, we dissected the natural intraspecific variability of tocochromanols in tomato fruits and genetically engineered their biosynthetic pathway. These analyses allowed the identification of a total of 25 QTL interspersed across the genome pinpointing the chorismate-tyrosine pathway as a regulatory hub controlling the supply of the aromatic head group for tocochromanol biosynthesis. To validate the link between the chorismate-tyrosine pathway and VTE, we engineered tomato plants to bypass the pathway at the arogenate branchpoint. Transgenic tomatoes showed moderate increments in tocopherols (up to ~ 20%) and a massive accumulation of tocotrienols (up to ~ 3,400%). Gene expression analyses of these plants reveal a trade-off between VTE and natural variation in chorismate metabolism explained by transcriptional reprogramming of specific structural genes of the pathway. By restoring the accumulation of α-t3 (alfa tocotrienols) in fruits, the plants produced here are of high pharmacological and nutritional interest.

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Language(s): eng - English
 Dates: 2020-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: URI: https://doi.org/10.1111/tpj.15077
 Degree: -

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Title: The Plant Journal
  Other : Plant J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1

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Title: The Plant Journal
Source Genre: Journal
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Publ. Info: John Wiley & Sons, Ltd
Pages: - Volume / Issue: n/a (n/a) Sequence Number: - Start / End Page: - Identifier: ISBN: 0960-7412