English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  ELAV and FNE Determine Neuronal Transcript Signatures through EXon-Activated Rescue

Carrasco, J., Rauer, M., Hummel, B., Grzejda, D., Alfonso-Gonzalez, C., Lee, Y., et al. (2020). ELAV and FNE Determine Neuronal Transcript Signatures through EXon-Activated Rescue. Molecular Cell, 80, 156-163. doi:10.1016/j.molcel.2020.09.011.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Carrasco et al.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
Carrasco et al.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
Online ahead of print

Creators

show
hide
 Creators:
Carrasco, Judit1, Author
Rauer, Michael1, Author              
Hummel, Barbara1, Author
Grzejda, Dominika1, Author
Alfonso-Gonzalez, Carlos1, Author
Lee, Yeon2, Author
Wang, Qingqing2, Author
Puchalska, Monika3, Author
Mittler, Gerhard3, Author              
Hilgers, Valérie1, Author              
Affiliations:
1Department Independent Research Groups, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243640              
2External Organizations, ou_persistent22              
3Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

Content

show
hide
Free keywords: 3′ UTR; Drosophila; ELAV proteins; EXAR; alternative polyadenylation; alternative splicing; functional activation; mRNA processing; mini-exon; neuron
 Abstract: The production of alternative RNA variants contributes to the tissue-specific regulation of gene expression. In the animal nervous system, a systematic shift toward distal sites of transcription termination produces transcript signatures that are crucial for neuron development and function. Here, we report that, in Drosophila, the highly conserved protein ELAV globally regulates all sites of neuronal 3' end processing and directly binds to proximal polyadenylation sites of target mRNAs in vivo. We uncover an endogenous strategy of functional gene rescue that safeguards neuronal RNA signatures in an ELAV loss-of-function context. When not directly repressed by ELAV, the transcript encoding the ELAV paralog FNE acquires a mini-exon, generating a new protein able to translocate to the nucleus and rescue ELAV-mediated alternative polyadenylation and alternative splicing. We propose that exon-activated functional rescue is a more widespread mechanism that ensures robustness of processes regulated by a hierarchy, rather than redundancy, of effectors.

Details

show
hide
Language(s): eng - English
 Dates: 2020-10-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2020.09.011
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 80 Sequence Number: - Start / End Page: 156 - 163 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929