English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Autonomous directional motion of actin-containing cell-sized droplets

Haller, B., Jahnke, K., Weiss, M., Göpfrich, K., Platzman, I., & Spatz, J. P. (2021). Autonomous directional motion of actin-containing cell-sized droplets. Advanced Intelligent Systems, 3(5): 2000190, pp. 1-8. doi:10.1002/aisy.202000190.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
AdvIntelligentSyst_3_2020_2000190.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl1.pdf (Supplementary material), 486KB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl2.avi (Supplementary material), 4MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl2.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl3.avi (Supplementary material), 3MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl3.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl4.avi (Supplementary material), 3MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl4.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl5.avi (Supplementary material), 3MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl5.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvIntelligentSyst_3_2020_2000190_Suppl6.avi (Supplementary material), 4MB
 
File Permalink:
-
Name:
AdvIntelligentSyst_3_2020_2000190_Suppl6.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Haller, Barbara1, Author              
Jahnke, Kevin, Author
Weiss, Marian1, Author              
Göpfrich, Kerstin, Author
Platzman, Ilia1, Author              
Spatz, Joachim Pius1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: actin; droplet-based microfluidics; Marangoni effect; motility; ; surfactants; synthetic biology
 Abstract: Cell motility is potentially the most apparent distinction of living matter, serving an essential purpose in single cells and multicellular organisms alike. Thus, the bottom‐up reconstitution of autonomous motion of cell‐sized compartments remains an exciting but challenging goal. Herein, actin‐driven Marangoni flows are engineered to generate rotational and translational motility of surfactant‐stabilized emulsion droplets. The interaction between actin filaments and the negatively charged block‐copolymer Krytox is identified as the driving force for Marangoni flows at the droplet interface. Tuning the actin–Krytox interplay, sustained autonomous unidirectional droplet rotation with 1.7 rot h−1 is achieved. Ultimately, this rotational motion is transformed into a translational rolling motion by introducing interactions between the droplets and the surface of the observation chamber. Accordingly, translational motility of actin‐containing droplets at velocities of 0.061 ± 0.014 μm s−1 is reported herein and an overall displacement of several hundreds of micrometers within 30 min is observed. These self‐propelled systems with biologically active molecules demonstrate how motility could be implemented for synthetic cells.

Details

show
hide
Language(s): eng - English
 Dates: 2020-09-222020-08-122020-11-162021-05-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/aisy.202000190
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Advanced Intelligent Systems
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH Verlag
Pages: - Volume / Issue: 3 (5) Sequence Number: 2000190 Start / End Page: 1 - 8 Identifier: ISSN: 2640-4567
CoNE: https://pure.mpg.de/cone/journals/resource/2640-4567