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  The promise and the challenges of cryo-electron tomography

Turk, M., & Baumeister, W. (2020). The promise and the challenges of cryo-electron tomography. FEBS Letters, 594(20), 3243-3261. doi:10.1002/1873-3468.13948.

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© 2020 The Authors. Open access funding enabled and organized by Projekt DEAL.

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 Creators:
Turk, Martin1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: TILT-SERIES ALIGNMENT; HERPES-SIMPLEX-VIRUS; IN-SITU STRUCTURE; CRYO-EM; 3-DIMENSIONAL RECONSTRUCTION; MACROMOLECULAR COMPLEXES; MOLECULAR ARCHITECTURE; 3D RECONSTRUCTION; PHASE PLATE; ELECTRONBiochemistry & Molecular Biology; Biophysics; Cell Biology; cellular structural biology; correlative light‐ electron microscopy; cryo‐ electron tomography; image processing workflow; sample preparation workflows; structural biology in situ;
 Abstract: Structural biologists have traditionally approached cellular complexity in a reductionist manner in which the cellular molecular components are fractionated and purified before being studied individually. This 'divide and conquer' approach has been highly successful. However, awareness has grown in recent years that biological functions can rarely be attributed to individual macromolecules. Most cellular functions arise from their concerted action, and there is thus a need for methods enabling structural studies performed in situ, ideally in unperturbed cellular environments. Cryo-electron tomography (Cryo-ET) combines the power of 3D molecular-level imaging with the best structural preservation that is physically possible to achieve. Thus, it has a unique potential to reveal the supramolecular architecture or 'molecular sociology' of cells and to discover the unexpected. Here, we review state-of-the-art Cryo-ET workflows, provide examples of biological applications, and discuss what is needed to realize the full potential of Cryo-ET.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000581179600001
DOI: 10.1002/1873-3468.13948
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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 594 (20) Sequence Number: - Start / End Page: 3243 - 3261 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501