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  Design of sealable custom-shaped cell mimicries based on self-assembled monolayers on CYTOP polymer

Eto, H., Soga, N., Franquelim, H. G., Glock, P., Khmelinskaia, A., Kai, L., et al. (2019). Design of sealable custom-shaped cell mimicries based on self-assembled monolayers on CYTOP polymer. ACS Applied Materials and Interfaces, 11(24), 21372-21380. doi:10.1021/acsami.9b05073.

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 Creators:
Eto, Hiro1, Author              
Soga, Naoki2, Author
Franquelim, Henri G.1, Author              
Glock, Philipp1, Author              
Khmelinskaia, Alena1, Author              
Kai, Lei1, Author
Heymann, Michael1, Author              
Noji, Hiroyuki2, Author
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              
2External Organizations, ou_persistent22              

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Free keywords: micropatterning bottom-up biology lab-on-a-chip synthetic biology self-assembled monolayers lipids polymer CYTOP
 Abstract: In bottom-up synthetic biology, one of the major methodological challenges is to provide reaction spaces that mimic biological systems with regard to topology and surface functionality. Of particular interest are cell- or organelle-shaped membrane compartments, as many protein functions unfold at lipid interfaces. However, shaping artificial cell systems using materials with non-intrusive physicochemical properties, while maintaining flexible lipid interfaces relevant to the reconstituted protein systems, is not straightforward. Herein, we develop micropatterned chambers from CYTOP, a less commonly used polymer with good chemical resistance and a refractive index matching that of water. By forming a self-assembled lipid monolayer on the polymer surface, we dramatically increased the biocompatibility of CYTOP-fabricated systems. The phospholipid interface provides an excellent passivation layer to prevent protein adhesion to the hydrophobic surface, and we succeeded in cell-free protein synthesis inside the chambers. Importantly, the chambers could be sealed after loading by a lipid monolayer, providing a novel platform to study encapsulated systems. We successfully reconstituted pole-to-pole oscillations of the Escherichia coli MinDE system, which responds dramatically to compartment geometry. Furthermore, we present a simplified fabrication of our artificial cell compartments via replica molding, making it a readily accessible technique for standard cleanroom facilities.

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 Dates: 2019
 Publication Status: Published online
 Pages: -
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 Identifiers: DOI: 10.1021/acsami.9b05073
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Project name : project A09 of the SFB1032 “Nanoagents for the spatiotemporal control of molecular and cellular reactions”
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Funding organization : DFG

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Title: ACS Applied Materials and Interfaces
  Other : ACS Applied Materials & Interfaces
  Abbreviation : ACS Appl. Mater. Interfaces
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 11 (24) Sequence Number: - Start / End Page: 21372 - 21380 Identifier: ISSN: 1944-8244
CoNE: https://pure.mpg.de/cone/journals/resource/1944-8244