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  Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus

Mann, A. E., Fellows Yates, J. A., Fagernäs, Z., Austin, R. M., Nelson, E. A., & Hofman, C. A. (2023). Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus. Quaternary International, 653–654: 2020.11.019, pp. 33-46. doi:10.1016/j.quaint.2020.11.019.

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 Urheber:
Mann, Allison E., Autor
Fellows Yates, James A.1, Autor           
Fagernäs, Zandra1, Autor           
Austin, Rita M., Autor
Nelson, Elizabeth A.1, Autor           
Hofman, Courtney A., Autor
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              

Inhalt

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Schlagwörter: Diet, Dental calculus, Metagenomics, Ancient DNA
 Zusammenfassung: Dental calculus and other preserved microbiome substrates are an attractive target for dietary reconstruction in past populations through a variety of physical, chemical, and molecular means. Recently, studies have attempted to reconstruct diet from archaeological dental calculus using archaeogenetic techniques. While dental calculus may provide a relatively stable environment for DNA preservation, the detection of plants and animals possibly consumed by an individual through DNA analysis is primarily hindered by microbial richness and incomplete reference databases. Moreover, high genomic similarity within eukaryotic groups

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Sprache(n): eng - English
 Datum: 2020-08-032020-11-122020-11-192023-04-20
 Publikationsstatus: Erschienen
 Seiten: 14
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: 1. Introduction
2. Materials and methods
3. Results
3.1. Effects of low read counts on damage patterns
3.2. Identification of dietary reads from synthetic datasets
3.3. Identification of dietary reads from real datasets
4. Discussion and conclusions
4.1. Checklist for authors and reviewers
4.1.1. Field/storage contamination
4.1.2. Is the laboratory methodology suitable for aDNA authentication?
4.1.3. Is the database and pipeline suitable?
4.1.4. Is the taxonomic resolution analyzed sufficient for reliable interpretation?
4.1.5. Have unexpected taxa been reported and evaluated?
4.1.6. Have laboratory controls been evaluated alongside samples?
4.1.7. Has evidence of aDNA authenticity been provided?
4.1.8. Is there other evidence supporting that dietary organisms were
consumed?
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.quaint.2020.11.019
Anderer: shh2769
 Art des Abschluß: -

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Titel: Quaternary International
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Oxford : Pergamon
Seiten: - Band / Heft: 653–654 Artikelnummer: 2020.11.019 Start- / Endseite: 33 - 46 Identifikator: ISSN: 1040-6182
CoNE: https://pure.mpg.de/cone/journals/resource/954925588348