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  Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus

Mann, A. E., Fellows Yates, J. A., Fagernäs, Z., Austin, R. M., Nelson, E. A., & Hofman, C. A. (2020). Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus. Quaternary International. doi:10.1016/j.quaint.2020.11.019.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-73CB-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-73CE-0
Genre: Journal Article

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(last checked: Nov. 2020)
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(last checked: Nov. 2020)

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 Creators:
Mann, Allison E., Author
Fellows Yates, James A.1, Author              
Fagernäs, Zandra1, Author              
Austin, Rita M., Author
Nelson, Elizabeth A.1, Author              
Hofman, Courtney A., Author
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              

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Free keywords: Diet, Dental calculus, Metagenomics, Ancient DNA
 Abstract: Dental calculus and other preserved microbiome substrates are an attractive target for dietary reconstruction in past populations through a variety of physical, chemical, and molecular means. Recently, studies have attempted to reconstruct diet from archaeological dental calculus using archaeogenetic techniques. While dental calculus may provide a relatively stable environment for DNA preservation, the detection of plants and animals possibly consumed by an individual through DNA analysis is primarily hindered by microbial richness and incomplete reference databases. Moreover, high genomic similarity within eukaryotic groups

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Language(s): eng - English
 Dates: 2020-11-19
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: 1. Introduction
2. Materials and methods
3. Results
3.1. Effects of low read counts on damage patterns
3.2. Identification of dietary reads from synthetic datasets
3.3. Identification of dietary reads from real datasets
4. Discussion and conclusions
4.1. Checklist for authors and reviewers
4.1.1. Field/storage contamination
4.1.2. Is the laboratory methodology suitable for aDNA authentication?
4.1.3. Is the database and pipeline suitable?
4.1.4. Is the taxonomic resolution analyzed sufficient for reliable interpretation?
4.1.5. Have unexpected taxa been reported and evaluated?
4.1.6. Have laboratory controls been evaluated alongside samples?
4.1.7. Has evidence of aDNA authenticity been provided?
4.1.8. Is there other evidence supporting that dietary organisms were
consumed?
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.quaint.2020.11.019
Other: shh2769
 Degree: -

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Title: Quaternary International
Source Genre: Journal
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Publ. Info: Oxford : Pergamon
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1040-6182
CoNE: https://pure.mpg.de/cone/journals/resource/954925588348