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  A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species

Zhang, Y., Chen, M., Siemiatkowska, B., Toleco, M., Jing, Y., Strotmann, V., et al. (2020). A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species. Plant Communications, 1(5): 100028. Retrieved from http://www.sciencedirect.com/science/article/pii/S2590346220300092.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-7AB6-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-7AB7-2
Genre: Journal Article

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 Creators:
Zhang, YJ1, Author              
Chen, Moxian2, Author
Siemiatkowska, B.1, Author              
Toleco, M.R.1, Author              
Jing, Y.1, Author              
Strotmann, Vivien2, Author
Zhang, Jianghua2, Author
Stahl, Yvonne2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2external, ou_persistent22              

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Free keywords: transient expression, agro-infiltration, subcellular localization, protein-protein interaction
 Abstract: Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
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Title: Plant Communications
Source Genre: Journal
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Pages: 100028 Volume / Issue: 1 (5) Sequence Number: 100028 Start / End Page: - Identifier: ISBN: 2590-3462