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  Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast

Strelnikova, N., Sauter, N., Guizar-Sicairos, M., Gollner, M., Diaz, A., Delivani, P., et al. (2017). Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast. Scientific Reports, 7: 13775. doi:10.1038/s41598-017-13175-9.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-614B-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-8728-C
Genre: Journal Article

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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
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 Creators:
Strelnikova, Natalja1, Author
Sauter, Nora1, Author
Guizar-Sicairos, Manuel1, Author
Gollner, Michael1, Author
Diaz, Ana1, Author
Delivani, Petrina1, Author
Chacon, Mariola1, Author
Tolic, Iva M.1, Author
Zaburdaev, Vasily2, 3, Author           
Pfohl, Thomas1, Author
Affiliations:
1external, ou_persistent22              
2External Organizations, ou_persistent22              
3Max Planck Institute for the Physics of Complex Systems, ou_persistent22              

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 Abstract: Seeing physiological processes at the nanoscale in living organisms without labeling is an ultimate goal in life sciences. Using X-ray ptychography, we explored in situ the dynamics of unstained, living fission yeast Schizosaccharomyces pombe cells in natural, aqueous environment at the nanoscale. In contrast to previous X-ray imaging studies on biological matter, in this work the eukaryotic cells were alive even after several ptychographic X-ray scans, which allowed us to visualize the chromatin motion as well as the autophagic cell death induced by the ionizing radiation. The accumulated radiation of the sequential scans allowed for the determination of a characteristic dose of autophagic vacuole formation and the lethal dose for fission yeast. The presented results demonstrate a practical method that opens another way of looking at living biological specimens and processes in a time-resolved label-free setting.

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Language(s): eng - English
 Dates: 2017-10-232017-10-23
 Publication Status: Issued
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 Identifiers: ISI: 000413400500050
DOI: 10.1038/s41598-017-13175-9
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Title: Scientific Reports
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 13775 Start / End Page: - Identifier: ISSN: 2045-2322