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  Analysis of Na+-D-glucose cotransporter and other renal brush border proteins in human urine

Birk, H.-W., Piberhofer, S., Schütterle, G., Haase, W., Koetting, J., & Koepsell, H. (1991). Analysis of Na+-D-glucose cotransporter and other renal brush border proteins in human urine. Kidney International, 40(5), 823-837. doi:10.1038/ki.1991.282.

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Birk, Horst-Walter1, Author
Piberhofer, Stefan 1, Author
Schütterle, Georg1, Author
Haase, Winfried2, Author           
Koetting, Jochem3, Author           
Koepsell, Hermann2, Author           
Affiliations:
1Medizinische Klinik II, Justus-Liebig-Universität Giessen, 6300 Giessen, Germany, ou_persistent22              
2Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
3Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: Analysis of Na+-D-glucose cotransporter and other renal brush border proteins in human urine. A sensitive quantitative radioimmunoassay is described by which different antigens in the urine can be assayed simultaneously. Urinary excretion of three proteins from proximal tubules was compared: 1) the Na+-D-glucose cotransporter from brush border membranes and subapical vesicles; 2) a kidney-specific hydrophobic Mr 400,000 polypeptide from intermicroviUar invaginations and subapical vesicles; and 3) villin from microvilli cores. In the normal urine about 50% of the excreted Na+-D-glucose cotransporter and villin, and about 25% of the Mr 400,000 polypeptide was associated with brush border membrane vesicles, whereas trie remaining fractions of the three proteins formed small sedimentable aggregates which contained some cholesterol and fatty acids but no phospholipids. The normal urinary excretion of the Na+-D-glucose cotransporter was correlated with that of villin and the Mr 400,000 polypeptide. The data show that membrane proteins from the proximal tubule are excreted by the shedding of different brush border membrane areas. They suggest that some microvilli are released in total, and that a large fraction of the brush border membrane proteins is excreted without being associated with a phospholipid bilayer. In an attempt to define protein excretion patterns during kidney malfunctions, the excretion of brush border membrane proteins was analyzed after one intravenous injection of the X-ray contrast medium, iopamidol. No change in villin excretion was observed, but a reversible increase in the excretion of brush border membrane proteins was found in patients without diabetes. With diabetes a more pronounced iopamidol effect on the excretion of brush border membrane proteins and a significant increase in the excretion of villin was observed.

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Language(s): eng - English
 Dates: 1991-07-011990-08-221991-07-022015-12-181991-11
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/ki.1991.282
 Degree: -

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Title: Kidney International
Source Genre: Journal
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Publ. Info: New York, NY : Elsevier ; International Society of Nephrology
Pages: - Volume / Issue: 40 (5) Sequence Number: - Start / End Page: 823 - 837 Identifier: ISSN: 0085-2538
CoNE: https://pure.mpg.de/cone/journals/resource/954927608814_1