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  Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease

Olah, M., Menon, V., Habib, N., Taga, M. F., Ma, Y., Yung, C. J., et al. (2020). Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease. Nature Communications, 11, 1-18. doi:10.1038/s41467-020-19737-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-85C5-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-AADF-F
Genre: Journal Article

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Online ahead of print

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 Creators:
Olah, Marta1, Author
Menon, Vilas1, Author
Habib, Naomi1, Author
Taga, Mariko F1, Author
Ma, Yiyi1, Author
Yung, Christina J1, Author
Cimpean, Maria1, Author
Khairallah, Anthony1, Author
Coronas-Samano, Guillermo1, Author
Sankowski, Roman1, Author
Grün, Dominic2, Author              
Kroshilina, Alexandra A1, Author
Dionne, Danielle1, Author
Sarkis, Rani A1, Author
Cosgrove, Garth R1, Author
Helgager, Jeffrey1, Author
Golden, Jeffrey A1, Author
Pennell, Page B1, Author
Prinz, Marco1, Author
Vonsattel, Jean Paul G1, Author
Teich, Andrew F1, AuthorSchneider, Julie A1, AuthorBennett, David A1, AuthorRegev, Aviv1, AuthorElyaman, Wassim1, AuthorBradshaw, Elizabeth M1, AuthorJager, Philip L De1, Author more..
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642              

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 Abstract: The extent of microglial heterogeneity in humans remains a central yet poorly explored question in light of the development of therapies targeting this cell type. Here, we investigate the population structure of live microglia purified from human cerebral cortex samples obtained at autopsy and during neurosurgical procedures. Using single cell RNA sequencing, we find that some subsets are enriched for disease-related genes and RNA signatures. We confirm the presence of four of these microglial subpopulations histologically and illustrate the utility of our data by characterizing further microglial cluster 7, enriched for genes depleted in the cortex of individuals with Alzheimer’s disease (AD). Histologically, these cluster 7 microglia are reduced in frequency in AD tissue, and we validate this observation in an independent set of single nucleus data. Thus, our live human microglia identify a range of subtypes, and we prioritize one of these as being altered in AD.

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Language(s): eng - English
 Dates: 2020-11-30
 Publication Status: Published in print
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-020-19737-2
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 Sequence Number: - Start / End Page: 1 - 18 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723