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  Environmental Liquid Cell Technique for Improved Electron Microscopic Imaging of Soft Matter in Solution

Azim, S., Bultema, L., de Kock, M., Osorio-Blanco, E. R., Calderón, M., Gonschior, J., et al. (2021). Environmental Liquid Cell Technique for Improved Electron Microscopic Imaging of Soft Matter in Solution. Microscopy and Microanalysis, 27(1), 44-53. doi:10.1017/S1431927620024654.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-87FC-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0008-0426-9
Genre: Journal Article

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https://dx.doi.org/10.1017/S1431927620024654 (Publisher version)
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 Creators:
Azim, S.1, Author              
Bultema, L.1, Author              
de Kock, M.1, 2, Author              
Osorio-Blanco, E. R.3, Author
Calderón, M.4, 5, Author
Gonschior, J.1, Author              
Leimkohl, J.-P.6, Author              
Tellkamp, F.6, Author              
Bücker, R.1, Author              
Schulz, E.-C.1, Author              
Keskin, S.7, Author
de Jonge, N.7, 8, Author
Kassier, G.1, Author              
Miller, R. J. D.1, 9, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg, ou_persistent22              
3Freie Universität Berlin, Institute of Chemistry and Biochemistry, ou_persistent22              
4POLYMAT & Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country UPV/EHU, ou_persistent22              
5IKERBASQUE, Basque Foundation for Science, ou_persistent22              
6Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
7INM–Leibniz Institute for New Materials, ou_persistent22              
8Department of Physics, Saarland University, ou_persistent22              
9Departments of Chemistry and Physics, University of Toronto, ou_persistent22              

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Free keywords: controlled nm liquid layer thickness, electron microscopy, environmental liquid cell, in-liquid imaging, structural dynamics
 Abstract: Liquid-phase transmission electron microscopy is a technique for simultaneous imaging of the structure and dynamics of specimens in a liquid environment. The conventional sample geometry consists of a liquid layer tightly sandwiched between two Si3N4 windows with a nominal spacing on the order of 0.5 μm. We describe a variation of the conventional approach, wherein the Si3N4 windows are separated by a 10-μm-thick spacer, thus providing room for gas flow inside the liquid specimen enclosure. Adjusting the pressure and flow speed of humid air inside this environmental liquid cell (ELC) creates a stable liquid layer of controllable thickness on the bottom window, thus facilitating high-resolution observations of low mass-thickness contrast objects at low electron doses. We demonstrate controllable liquid thicknesses in the range 160 ± 34 to 340 ± 71 nm resulting in corresponding edge resolutions of 0.8 ± 0.06 to 1.7 ± 0.8 nm as measured for immersed gold nanoparticles. Liquid layer thickness 40 ± 8 nm allowed imaging of low-contrast polystyrene particles. Hydration effects in the ELC have been studied using poly-N-isopropylacrylamide nanogels with a silica core. Therefore, ELC can be a suitable tool for in situ investigations of liquid specimens.

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Language(s): eng - English
 Dates: 2020-09-182020-03-262020-10-262020-12-072021-02
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1017/S1431927620024654
 Degree: -

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Title: Microscopy and Microanalysis
  Abbreviation : Microsc. Microanal.
Source Genre: Journal
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Publ. Info: New York, NY : Cambridge University Press
Pages: - Volume / Issue: 27 (1) Sequence Number: - Start / End Page: 44 - 53 Identifier: ISSN: 1431-9276
CoNE: https://pure.mpg.de/cone/journals/resource/991042731793414