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  DNA‐Barcoded Fluorescence Microscopy for Spatial Omics

Schueder, F., Unterauer, E. M., Ganji, M., & Jungmann, R. (2020). DNA‐Barcoded Fluorescence Microscopy for Spatial Omics. Proteomics, 20(23, SI): 1900368. doi:10.1002/pmic.201900368.

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Genre: Journal Article
Subtitle : Review

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pmic.201900368.pdf (Publisher version), 4MB
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© 2020 The Authors. Open access funding enabled and organized by Projekt DEAL.

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 Creators:
Schueder, Florian1, Author              
Unterauer, Eduard M.1, Author              
Ganji, Mahipal1, Author              
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: SUPERRESOLUTION MICROSCOPY; SINGLE CELLS; RESOLUTION; TISSUES; PAINT; LIMITBiochemistry & Molecular Biology; DNA nanotechnology; fluorescence microscopy; high‐ content imaging; multiplexing; super‐ resolution microscopy;
 Abstract: Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an almost routine basis, coupling this high content to spatial information in a single cell and tissue context is challenging. Here, current implementations of spatial omics are discussed and recent developments in the field of DNA-barcoded fluorescence microscopy are reviewed. Light is shed on the potential of DNA-based imaging techniques to provide a comprehensive toolbox for spatial genomics and transcriptomics and discuss current challenges, which need to be overcome on the way to spatial proteomics using high-resolution fluorescence microscopy.

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Language(s): eng - English
 Dates: 2020-12
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000583773800001
DOI: 10.1002/pmic.201900368
 Degree: -

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Project name : Grant Numbers: DFG JU 2957/1‐1, SFB 1032 (Nanoagents for the spatiotemporal control of molecular and cellular reactions, project A11)
Grant ID : -
Funding program : Emmy Noether Fellowship
Funding organization : Deutsche Forschungsgemeinschaft
Project name : ERC Starting Grant (MolMap)
Grant ID : 680241
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : Marie Skłodowska‐Curie Fellowship
Grant ID : 796606
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Proteomics
Source Genre: Journal
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Publ. Info: Weinheim : WILEY-VCH
Pages: - Volume / Issue: 20 (23, SI) Sequence Number: 1900368 Start / End Page: - Identifier: ISSN: 1615-9853
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000294310