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  Association of Bcl-2 with misfolded prion protein is linked to the toxic potential of cytosolic PrP

Rambold, A., Miesbauer, M., Rapaport, D., Bartke, T., Baier, M., Winklhofer, K. F., et al. (2006). Association of Bcl-2 with misfolded prion protein is linked to the toxic potential of cytosolic PrP. Molecular and Cellular Biology (Washington, DC), 17, 3356-3368. doi:10.1091/mbc.e06-01-0083.

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Rambold et al. 2006.pdf (Publisher version), 2MB
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Rambold, Angelika1, Author              
Miesbauer, Margit2, Author
Rapaport, Doron2, Author
Bartke, Till2, Author
Baier, Michael2, Author
Winklhofer, Konstanze F2, Author
Tatzelt, Jörg2, Author
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1Department of Developmental Immunobiology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243650              
2External Organizations, ou_persistent22              

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 Abstract: Protein misfolding is linked to different neurodegenerative disorders like Alzheimer’s disease, polyglutamine, and prion diseases. We investigated the cytotoxic effects of aberrant conformers of the prion protein (PrP) and show that toxicity is specifically linked to misfolding of PrP in the cytosolic compartment and involves binding of PrP to the anti-apoptotic protein Bcl-2. PrP targeted to different cellular compartments, including the cytosol, nucleus, and mitochondria, adopted a misfolded and partially proteinase K–resistant conformation. However, only in the cytosol did the accumulation of misfolded PrP induce apoptosis. Apoptotic cell death was also induced by two pathogenic mutants of PrP, which are partially localized in the cytosol. A mechanistic analysis revealed that the toxic potential is linked to an internal domain of PrP (amino acids 115–156) and involves coaggregation of cytosolic PrP with Bcl-2. Increased expression of the chaperones Hsp70 and Hsp40 prevented the formation of PrP/Bcl-2 coaggregates and interfered with PrP-induced apoptosis. Our study reveals a compartment-specific toxicity of PrP misfolding that involves coaggregation of Bcl-2 and indicates a protective role of molecular chaperones.

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Language(s): eng - English
 Dates: 2006-08
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1091/mbc.e06-01-0083
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Title: Molecular and Cellular Biology (Washington, DC)
  Other : Mol Cell Biol
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 3356 - 3368 Identifier: ISSN: 0270-7306
CoNE: https://pure.mpg.de/cone/journals/resource/954925502188