English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Drift and Behavior of E. coli Cells

Micali, G., Colin, R., Sourjik, V., & Endres, R. (2017). Drift and Behavior of E. coli Cells. Biophysical Journal, 113(11), 2321-2325. doi:10.1016/j.bpj.2017.09.031.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Micali, G., Author
Colin, R.1, Author           
Sourjik, V.2, 3, Author           
Endres, R., Author
Affiliations:
1Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266288              
2Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266309              
3Center for Synthetic Microbiology (SYNMIKRO), ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Chemotaxis of the bacterium Escherichia coli is well understood in shallow chemical gradients, but its swimming behavior remains difficult to interpret in steep gradients. By focusing on single-cell trajectories from simulations, we investigated the dependence of the chemotactic drift velocity on attractant concentration in an exponential gradient. Whereas maxima of the average drift velocity can be interpreted within analytical linear-response theory of chemotaxis in shallow gradients, limits in drift due to steep gradients and finite number of receptor-methylation sites for adaptation go beyond perturbation theory. For instance, we found a surprising pinning of the cells to the concentration in the gradient at which cells run out of methylation sites. To validate the positions of maximal drift, we recorded single-cell trajectories in carefully designed chemical gradients using microfluidics.

Details

show
hide
Language(s):
 Dates: 2017-12-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Internal
 Identifiers: eDoc: 735371
ISI: 000417264300003
DOI: 10.1016/j.bpj.2017.09.031
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 113 (11) Sequence Number: - Start / End Page: 2321 - 2325 Identifier: ISSN: 0006-3495