English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Multiple sources of slow activity fluctuations in a bacterial chemosensory network

Colin, R., Rosazza, C., Vaknin, A., & Sourjik, V. (2017). Multiple sources of slow activity fluctuations in a bacterial chemosensory network. eLife, 6:e26796. doi:10.7554/eLife.26796.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Colin, Remy1, Author           
Rosazza, Christelle, Author
Vaknin, Ady, Author
Sourjik, Victor2, 3, Author           
Affiliations:
1Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266288              
2Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266309              
3Center for Synthetic Microbiology (SYNMIKRO), ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Cellular networks are intrinsically subject to stochastic fluctuations, but analysis of the resulting noise remained largely limited to gene expression. The pathway controlling chemotaxis of Escherichia coli provides one example where posttranslational signaling noise has been deduced from cellular behavior. This noise was proposed to result from stochasticity in chemoreceptor methylation, and it is believed to enhance environment exploration by bacteria. Here we combined single-cell FRET measurements with analysis based on the fluctuation-dissipation theorem (FDT) to characterize origins of activity fluctuations within the chemotaxis pathway. We observed surprisingly large methylation-independent thermal fluctuations of receptor activity, which contribute to noise comparably to the energy-consuming methylation dynamics. Interactions between clustered receptors involved in amplification of chemotactic signals are also necessary to produce the observed large activity fluctuations. Our work thus shows that the high response sensitivity of this cellular pathway also increases its susceptibility to noise, from thermal and out-of-equilibrium processes.

Details

show
hide
Language(s): eng - English
 Dates: 2017-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 735488
DOI: 10.7554/eLife.26796
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 6:e26796 Start / End Page: - Identifier: -