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  PREPARATION OF [FE]-HYDROGENASE FROM METHANOGENIC ARCHAEA

Shima, S., Schick, M., & Tamura, H. (2011). PREPARATION OF [FE]-HYDROGENASE FROM METHANOGENIC ARCHAEA. Methods in Enzymology, (494), 119-137. doi:10.1016/b978-0-12-385112-3.00007-x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-C2C7-D Version Permalink: https://hdl.handle.net/21.11116/0000-0009-43BC-8
Genre: Journal Article

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 Creators:
Shima, S.1, Author           
Schick, M.2, Author           
Tamura, H.1, Author           
Affiliations:
1Department-Independent Research Group Microbial Protein Structure, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266277              
2Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              

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 Abstract: [Fe]-hydrogenase is one of the three types of hydrogenases. This enzyme is found in many hydrogenotrophic methanogenic archaea and catalyzes the reversible hydride transfer from H(2) to methenyl-H(4)MPT(+) in methanogenesis from H(2) and CO(2). The enzyme harbors a unique iron-guanylyl pyridinol (FeGP) cofactor as a prosthetic group. Here, we describe the purification of [Fe]-hydrogenase from Methanothermobacter marburgensis, the isolation of the FeGP cofactor from the native holoenzyme, and the reconstitution of [Fe]-hydrogenase from the isolated FeGP cofactor and the heterologously produced apoenzyme.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 583300
ISI: 000289444900007
DOI: 10.1016/b978-0-12-385112-3.00007-x
 Degree: -

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Title: Methods in Enzymology
  Other : Methods Enzymol.
Source Genre: Journal
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Publ. Info: New York, NY : Academic Press
Pages: - Volume / Issue: (494) Sequence Number: - Start / End Page: 119 - 137 Identifier: ISSN: 0076-6879
CoNE: https://pure.mpg.de/cone/journals/resource/110975506069301