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  Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome

Albers, S., & Driessen, A. (2010). Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome. Archaea, 2, 145-149.

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 Creators:
Albers, S.1, Author           
Driessen, A., Author
Affiliations:
1Max Planck Research Group Molecular Biology of Archaea, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266317              

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Free keywords: lactose auxotrophy, selection
 Abstract: The construction of directed gene deletion mutants is an essential tool in molecular biology that allows functional studies on the role of genes in their natural environment. For hyperthermophilic archaea, it has been difficult to obtain a reliable system to construct such mutants. However, during the past years, systems have been developed for Thermococcus kodakarensis and two Sulfolobus species, S. acidocaldarius and derivatives of S. solfataricus 98/2. Here we describe an optimization of the method for integration of exogenous DNA into S. solfataricus PBL 2025, an S. solfataricus 98/2 derivative, based on lactose auxotrophy that now allows for routine gene inactivation.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 463316
 Degree: -

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Title: Archaea
Source Genre: Journal
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Pages: - Volume / Issue: 2 Sequence Number: - Start / End Page: 145 - 149 Identifier: -