English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins

Bulyha, I., Schmidt, C., Lenz, P., Jakovljevic, V., Höne, A., Maier, B., et al. (2009). Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins. Molecular Microbiology, 74(3), 691-706. doi:10.1111/j.1365-2958.2009.06891.x.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bulyha, Iryna1, Author           
Schmidt, Carmen1, Author           
Lenz, Peter, Author
Jakovljevic, Vladimir1, Author           
Höne, Andrea, Author
Maier, Berenike, Author
Hoppert, Michael, Author
Sogaard-Andersen, Lotte1, Author           
Affiliations:
1Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266305              

Content

show
hide
Free keywords: -
 Abstract: Type IV pili (T4P) are surface structures that undergo extension/retraction oscillations to generate cell motility. In Myxococcus xanthus, T4P are unipolarly localized and undergo pole-to-pole oscillations synchronously with cellular reversals. We investigated the mechanisms underlying these oscillations. We show that several T4P proteins localize symmetrically in clusters at both cell poles between reversals, and these clusters remain stationary during reversals. Conversely, the PilB and PilT motor ATPases that energize extension and retraction, respectively, localize to opposite poles with PilB predominantly at the piliated and PilT predominantly at the non-piliated pole, and these proteins oscillate between the poles during reversals. Therefore, T4P pole-to-pole oscillations involve the disassembly of T4P machinery at one pole and reassembly of this machinery at the opposite pole. Fluorescence recovery after photobleaching experiments showed rapid turnover of YFP-PilT in the polar clusters between reversals. Moreover, PilT displays bursts of accumulation at the piliated pole between reversals. These observations suggest that the spatial separation of PilB and PilT in combination with the noisy PilT accumulation at the piliated pole allow the temporal separation of extension and retraction. This is the first demonstration that the function of a molecular machine depends on disassembly and reassembly of its individual parts.

Details

show
hide
Language(s): eng - English
 Dates: 2009-10-06
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 438087
DOI: 10.1111/j.1365-2958.2009.06891.x
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Microbiology
  Alternative Title : Mol. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 74 (3) Sequence Number: - Start / End Page: 691 - 706 Identifier: -