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  A Conformational Change in the α‐subunit of Coatomer Induced by Ligand Binding to γ‐COP Revealed by Single‐pair FRET

Langer, J. D., Roth, C. M., Béthune, J., Stoops, E. H., Brügger, B., Herten, D.-P., et al. (2008). A Conformational Change in the α‐subunit of Coatomer Induced by Ligand Binding to γ‐COP Revealed by Single‐pair FRET. Traffic, 9(4), 597-607. doi:10.1111/j.1600-0854.2007.00697.x.

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Genre: Journal Article

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 Creators:
Langer, Julian David1, Author              
Roth, Christian M.2, Author
Béthune, Julien 1, Author
Stoops, Emily H.1, Author
Brügger, Britta1, Author
Herten, Dirk-Peter2, Author
Wieland, Felix T.1, Author
Affiliations:
1Heidelberg University Biochemistry Center, Heidelberg University, 69120 Heidelberg, Germany, ou_persistent22              
2CellNetworks/BIOQUANT, Heidelberg University, Im Neuenheimer Feld 267/BQ 0007, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: COPI; coatomer; fluorescence resonance energy transfer; p24 proteins; single‐molecule spectroscopy
 Abstract: Formation of transport vesicles involves polymerization of cytoplasmic coat proteins (COP). In COPI vesicle biogenesis, the heptameric complex coatomer is recruited to donor membranes by the interaction of multiple coatomer subunits with the budding machinery. Specific binding to the trunk domain of γ‐COP by the Golgi membrane protein p23 induces a conformational change that causes polymerization of the complex. Using single‐pair fluorescence resonance energy transfer, we find that this conformational change takes place in individual coatomer complexes, independent of each other, and that the conformational rearrangement induced in γ‐COP is transmitted within the complex to its α‐subunit. We suggest that capture of membrane protein machinery triggers cage formation in the COPI system.

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Language(s): eng - English
 Dates: 2007-05-042007-12-202007-12-252008-04-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Traffic
  Abbreviation : tra
Source Genre: Journal
 Creator(s):
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Publ. Info: Oxford, UK : Wiley-Blackwell
Pages: - Volume / Issue: 9 (4) Sequence Number: - Start / End Page: 597 - 607 Identifier: ISSN: 1600-0854
CoNE: https://pure.mpg.de/cone/journals/resource/1600-0854