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  To close or to collapse : the role of charges on membrane stability upon pore formation

Lira, R. d., Leomil, F. S., Melo, R. J., Riske, K. A., & Dimova, R. (2021). To close or to collapse: the role of charges on membrane stability upon pore formation. Advanced Science, 8(11): 2004068. doi:10.1002/advs.202004068.

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 Creators:
Lira, Rafael de1, Author              
Leomil, Fernanda S.C., Author
Melo, Renan J., Author
Riske, Karin A., Author
Dimova, Rumiana1, Author              
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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Free keywords: calcium, charged lipids, detergent, edge tension, electroporation, giant unlamellar vesicles, membrane solubilization
 Abstract: Resealing of membrane pores is crucial for cell survival. We study membrane surface charge and medium composition as defining regulators of membrane stability. Pores are generated by electric field or detergents. Giant vesicles composed of zwitterionic and negatively charged lipids mixed at varying ratios are subjected to a single strong electric pulse. Charged vesicles are prone to catastrophic collapse transforming them into tubular structures. The spectrum of destabilization responses includes the generation of long-living submicroscopic pores and partial vesicle bursting. The origin of these phenomena is related to the membrane edge tension, which governs pore closure. This edge tension significantly decreases as a function of the fraction of charged lipids. Destabilization of charged vesicles upon pore formation is universal – it is also observed with other poration stimuli. Disruption propensity is enhanced for membranes made of lipids with higher degree of unsaturation. It can be reversed by screening membrane charge in the presence of calcium ions. We interpret the observed findings in light of theories of stability and curvature generation and discuss mechanisms acting in cells to prevent total membrane collapse upon poration. Enhanced membrane stability is crucial for the success of electroporation-based technologies for cancer treatment and gene transfer.Competing Interest StatementThe authors have declared no competing interest.

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Language(s): eng - English
 Dates: 2021-03-082021
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1002/advs.202004068
DOI: 10.1101/2020.08.31.274860
BibTex Citekey: Lira2020.08.31.274860
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Title: Advanced Science
  Abbreviation : Adv. Sci.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 8 (11) Sequence Number: 2004068 Start / End Page: - Identifier: ISSN: 2198-3844

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Title: bioRxiv
Source Genre: Journal
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Publ. Info: Cold Spring Harbor : Cold Spring Harbor Laboratory
Pages: - Volume / Issue: - Sequence Number: 2020.08.31.274860 Start / End Page: - Identifier: ZDB: 2766415-6