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  Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

Li, H., Nazari, R., Abbey, B., Alvarez, R., Aquila, A., Ayyer, K., et al. (2020). Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source. Scientific Data, 7: 404. doi:10.1038/s41597-020-00745-2.

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Li, H.1, Author
Nazari, R.1, Author
Abbey, B.1, Author
Alvarez, R.1, Author
Aquila, A.1, Author
Ayyer, K.2, 3, Author           
Barty, A.1, Author
Berntsen, P.1, Author
Bielecki, J.1, Author
Pietrini, A.1, Author
Bucher, M.1, Author
Carini, G.1, Author
Chapman, H. N.1, Author
Contreras, A.1, Author
Daurer, B. J.1, Author
DeMirci, H.1, Author
Flűckiger, L.1, Author
Frank, M.1, Author
Hajdu, J.1, Author
Hantke, M. F.1, Author
Hogue, B. G.1, AuthorHosseinizadeh, A.1, AuthorHunter, M. S.1, AuthorJönsson, H. O.1, AuthorKirian, R. A.1, AuthorKurta, R. P.1, AuthorLoh, D.1, AuthorMaia, F. R. N. C.1, AuthorMancuso, A. P.1, AuthorMorgan, A. J.1, AuthorMcFadden, M.1, AuthorMuehlig, K.1, AuthorMunke, A.1, AuthorReddy, H. K. N.1, AuthorNettelblad, C.1, AuthorOurmazd, A.1, AuthorRose, M.1, AuthorSchwander, P.1, AuthorMarvin Seibert, M.1, AuthorSellberg, J. A.1, AuthorSierra, R G.1, AuthorSun, Z.1, AuthorSvenda, M.1, AuthorVartanyants, I. A.1, AuthorWalter, P.1, AuthorWestphal, D.1, AuthorWilliams, G.1, AuthorXavier, P. L.1, AuthorYoon, C. H.1, AuthorZaare, S.1, Author more..
Affiliations:
1external, ou_persistent22              
2Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3012829              
3Center for Free Electron Laser Science, ou_persistent22              

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 Abstract: Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

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 Dates: 2020-08-112020-10-292020-11-19
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41597-020-00745-2
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Grant ID : 701647
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : Use of the Linac Coherent Light Source, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. The research conducted at UWM was supported by the US Department of Energy, Office of Science, Basic Energy Sciences under award DE-SC0002164 (algorithm design and development), and by the US National Science Foundation under awards STC 1231306 (numerical trial models and data analysis) and 1551489 (underlying analytical models). This work was supported by the Cluster of Excellence “CUI: Advanced Imaging of Matter” of the Deutsche Forschungsgemeinschaft (DFG) - EXC 2056 - project ID 390715994. The US National Science Foundation Award 1231306. The NSF Science and Technology Center grant NSF-1231306 (Biology with X-ray Lasers, BioXFEL). NIH grant 5R01GM117342. Helmholtz Associations Initiative and Networking Fund and Russian Science Foundation (Grant No. HRSF-0002/18-41-06001). This project has recieved funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 701647. The Swedish Research Council (to J.H.), The Knut and Alice Wallenberg Foundation (to J.H.), The European Research Council (to J.H.), and the project “Structural dynamics of biomolecular systems (ELIBIO)” (NO. CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund (to JH). The project “Structural dynamics of biomolecular systems (ELIBIO)” (NO. CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund. The European Research Council—Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS). The Australian Research Council Centre of Excellence in Advanced Molecular Imaging (AMI).
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Title: Scientific Data
  Abbreviation : Sci. Data
Source Genre: Journal
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Publ. Info: London, United Kingdom : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 404 Start / End Page: - Identifier: ISSN: 2052-4463
CoNE: https://pure.mpg.de/cone/journals/resource/2052-4463