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  Temperature Dependence of the Krokinobacter rhodopsin 2 Kinetics

Eberhardt, P., Slavov, C., Sörmann, J., Bamann, C., Braun, M., & Wachtveitl, J. (2021). Temperature Dependence of the Krokinobacter rhodopsin 2 Kinetics. Biophysical Journal (Annual Meeting Abstracts), 120(3), 568-575. doi:10.1016/j.bpj.2020.12.011.

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 Creators:
Eberhardt, Peter1, Author
Slavov, Chavdar1, Author
Sörmann, Janina2, Author              
Bamann, Christian2, Author              
Braun, Markus1, Author
Wachtveitl, Josef1, Author
Affiliations:
1Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany, ou_persistent22              
2Emeritusgroup Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2253652              

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 Abstract: We investigated the temperature dependent kinetics of the light-driven Na+ pump Krokinobacter rhodopsin 2 (KR2) at Na+ pumping conditions. The recorded μs flash photolysis data were subjected to detailed global target analysis, employing Eyring constraints and spectral decomposition. The analysis resulted in the kinetic rates, the composition of the different photocycle equilibria and the spectra of the involved photointermediates. Our results show that with the temperature increase (from 10 to 40 °C) the overall photocycle duration is accelerated by a factor of six, with the L to M transition exhibiting an impressive 40-fold increase. It follows from the analysis that in KR2 the chromophore and the protein scaffold are more kinetically decoupled than in other microbial rhodopsins. We link this effect to the rigidity of the retinal protein environment. This kinetic decoupling should be considered in future studies and could potentially be exploited for fine-tuning biotechnological applications.

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Language(s): eng - English
 Dates: 2020-07-242020-12-072020-12-182021-02-02
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bpj.2020.12.011
Other: https://libkey.io/libraries/2385/10.1016/j.bpj.2020.12.011
BibTex Citekey: eberhardt_temperature_2020
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Title: Biophysical Journal (Annual Meeting Abstracts)
  Other : Biophys. J. (Annual Meeting Abstracts)
Source Genre: Journal
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Publ. Info: Bethesda, MD : Biophysical Society
Pages: - Volume / Issue: 120 (3) Sequence Number: - Start / End Page: 568 - 575 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117_1