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Abstract:
Electron cryocrystallography of precipitant-induced two-dimensional surface crystals of the neurospora plasma membrane H+-ATPase and tubular crystals of the sarcoplasmic reticulum Ca2+-ATPase has recently yielded structure maps for these ion transporters at a resolution of about 8 Å. The membrane-embedded regions of these closely related enzymes are similar, but the cytoplasmic regions appear to be significantly different.