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  Crystallization and preliminary X-ray analysis of the bacterial ATP-binding-cassette (ABC) protein MalK

Schmees, G., Höner zu Bentrup, K., Schneider, E., Vinzenz, D., & Ermler, U. (1999). Crystallization and preliminary X-ray analysis of the bacterial ATP-binding-cassette (ABC) protein MalK. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen), 55(1), 285-286. doi:10.1107/S0907444998008518.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-A2E1-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-A2E2-2
Genre: Journal Article

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 Creators:
Schmees, G.1, 2, Author
Höner zu Bentrup, K.2, 3, Author
Schneider, E. 1, 2, Author
Vinzenz, Daniela4, Author              
Ermler, Ulrich4, Author              
Affiliations:
1Institut für Biologie/Bakterienphysiologie, Humboldt-Universität zu Berlin, 10115 Berlin, Germany, ou_persistent22              
2Mikro­biologie, Universität Osnabrück, FB 5, 49069 Osnabrück, Germany, ou_persistent22              
3Molecular Microbiology, Medical School, Washington University, 6609 Euclid Avenue, St Louis, MO 63110, USA, ou_persistent22              
4Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: ATP-binding cassette protein (S. typhimurium)
 Abstract: The ATP-binding protein, MalK, of the bacterial ABC (ATP-binding-cassette) transport complex MalFGK2 provides the energy for the translocation of maltose and maltodextrins across the cytoplasmic membrane. The MalK protein from Salmonella typhimurium was overexpressed in Escherichia coli and crystallized by the hanging-drop method using (NH4)2SO4as a precipitant. The crystals belong to space group P6x22 (most probably x = 1 or 5) with cell dimensions a = 181.8 and c = 182.5 A, corresponding to three or four molecules per asymmetric unit. They diffract to a resolution of about 3 A on a synchrotron X-ray source and are suitable for structure determination.

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Language(s): eng - English
 Dates: 2007-09-271999-01-01
 Publication Status: Published in print
 Pages: 2
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S0907444998008518
PMID: 10089426
 Degree: -

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Title: Acta Crystallographica. Section D: Biological Crystallography (Copenhagen)
  Other : Acta Crystallogr D
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 55 (1) Sequence Number: - Start / End Page: 285 - 286 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619