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  Purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach

Dietrich, J., & Kühlbrandt, W. (1999). Purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach. FEBS Letters, 463(1-2), 97-102. doi:10.1016/S0014-5793(99)01609-9.

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 Creators:
Dietrich, Jens1, Author           
Kühlbrandt, Werner1, Author           
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Free keywords: Cytochrome b6f; Purification; Two-dimensional crystal; Spinach
 Abstract: The purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach is described. The preparation shows all spectroscopic characteristics of the pure complex. The electron transfer activity of 450±60 electrons per s is the highest in vitro activity reported to date. Using dimethyl sulfoxide (DMSO) as a solvent for the electron donor enhanced the performance and reproducibility of the assay. The high yield and the high activity of the protein make it an ideal candidate for biophysical and structural studies. Preliminary two-dimensional crystallization experiments yielded several different forms of two-dimensional and thin three-dimensional crystals, exhibiting varying degrees of order.

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Language(s): eng - English
 Dates: 1999-11-051999-10-131999-12-071999-12-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0014-5793(99)01609-9
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 463 (1-2) Sequence Number: - Start / End Page: 97 - 102 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501