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  Division and regrowth of phase-separated giant unilamellar vesicles

Dreher, Y., Jahnke, K., Bobkova, E., Spatz, J. P., & Göpfrich, K. (2021). Division and regrowth of phase-separated giant unilamellar vesicles. Angewandte Chemie International Edition, 60(19), 10661-10669. doi:10.1002/anie.202014174.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-A561-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-21FD-5
Genre: Journal Article

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 Creators:
Dreher, Yannik1, Author           
Jahnke, Kevin1, Author           
Bobkova, Elizaveta2, Author           
Spatz, Joachim P.1, Author           
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: DNA Structures; GUV division; Osmosis; Synthetic biology; Vesicles
 Abstract: Success in the bottom-up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase-separated giant unilamellar lipid vesicles (GUVs). We derive an analytical model based on the vesicle geometry which makes four quantitative predictions that we verify experimentally. We find that the osmolarity ratio required for division is sqrt(2), independent of the GUV size, while asymmetric division happens at lower osmolarity ratios. Remarkably, we show that a suitable osmolarity change can be triggered by water evaporation, enzymatic decomposition of sucrose or light-triggered uncaging of CMNB-fluorescein. The latter provides full spatio-temporal control, such that a target-GUV undergoes division whereas the surrounding GUVs remain unaffected. Finally, we grow phase-separated vesicles from single-phased vesicles by targeted fusion of the opposite lipid type with programmable DNA tags to enable subsequent division cycles.

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Language(s): eng - English
 Dates: 2020-12-132020-10-222020-12-142020-12-232021-05-21
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202014174
URI: https://pubmed.ncbi.nlm.nih.gov/33355974/
 Degree: -

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 60 (19) Sequence Number: - Start / End Page: 10661 - 10669 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851