Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Preparation of white resealable erythrocyte ghosts

Wood, P. G. (1987). Preparation of white resealable erythrocyte ghosts. In R. Green, & K. J. Widder (Eds.), Methods in Enzymology (pp. 271-280). Academic Press. doi:10.1016/0076-6879(87)49065-4.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0007-A5A8-1 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0007-A5A9-0
Genre: Buchkapitel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Wood, Phillip G.1, Autor           
Affiliations:
1Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Publisher Summary:
This chapter discusses that in biochemical labeling studies, in enzymatic digestion, and in optical studies of the membrane, the residual hemoglobin may cause an unwanted problem. Methods that deplete the membranes of all cytosolic materials through multiple washing steps generally form membranes that cannot be resealed tightly again. To overcome these problems, the following gel filtration procedure was developed. After collecting the membranes, it has been found convenient to reverse the flow to clear the column of the released hemoglobin. This is the fastest approach, since the hemoglobin front is down only about one-third the length of the column. The column may be cleaned by reverse flow with two to three bed volumes of isotonic saline, pH 7.6. The agarose should recover its white color. About once a week, depending on the frequency of use, the column is rinsed with two bed volumes of solution F to control bacterial growth.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2004-01-071987
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/0076-6879(87)49065-4
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Methods in Enzymology
  Untertitel : Drug and Enzyme Targeting Part B
Genre der Quelle: Reihe
 Urheber:
Green, Ralph, Herausgeber
Widder, Kenneth J. , Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: Academic Press
Seiten: 359 Band / Heft: 149 Artikelnummer: - Start- / Endseite: 271 - 280 Identifikator: ISBN: 978-0-12-182049-7
ISSN: 0076-6879