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  An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling

Oliver‐Cervelló, L., Martin‐Gómez, H., Reyes, L., Noureddine, F., Cavalcanti-Adam, E. A., Ginebra, M., et al. (2020). An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling. Advanced Healthcare Materials, 2001757, pp. 1-10. doi:10.1002/adhm.202001757.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-A58B-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-A58C-1
Genre: Journal Article

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AdvHealthcareMater_epub_2020_2001757.pdf (Any fulltext), 3MB
 
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AdvHealthcareMater_epub_2020_2001757_Suppl.pdf (Supplementary material), 483KB
 
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 Creators:
Oliver‐Cervelló, Lluís, Author
Martin‐Gómez, Helena, Author
Reyes, Leslie, Author
Noureddine, Fatima1, Author              
Cavalcanti-Adam, Elisabetta Ada2, Author              
Ginebra, Maria‐Pau, Author
Mas‐Moruno, Carlos, Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: DWIVA; RGD; biomimetic peptides; cell adhesion; cell differentiation; surface functionalization
 Abstract: Recreating the healing microenvironment is essential to regulate cell-material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However, current approaches relying on the use of GFs, such as bone morphogenetic protein 2 (BMP-2), entail clinical risks. Here, a biomimetic peptide integrating the RGD cell adhesive sequence and the osteogenic DWIVA motif derived from the wrist epitope of BMP-2 is presented. The approach offers the advantage of having a spatial control over the single binding of integrins and BMP receptors. Such multifunctional platform is designed to incorporate 3,4-dihydroxyphenylalanine to bind metallic oxides with high affinity in a one step process. Functionalization of glass substrates with the engineered peptide is characterized by physicochemical methods, proving a successful surface modification. The biomimetic interfaces significantly improve the adhesion of C2C12 cells, inhibit myotube formation, and activate the BMP-dependent signaling via p38. These effects are not observed on surfaces displaying only one bioactive motif, a mixture of both motifs or soluble DWIVA. These data prove the biological potential of recreating the ECM and engaging in integrin and GF crosstalk via molecular-based mimics.

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Language(s): eng - English
 Dates: 2020-12-022020-10-062020-12-18
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Advanced Healthcare Materials
Source Genre: Journal
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Publ. Info: Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: 2001757 Start / End Page: 1 - 10 Identifier: ISSN: 2192-2640
CoNE: https://pure.mpg.de/cone/journals/resource/2192-2640