English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Alkaline phosphatase delivery system based on calcium carbonate carriers for acceleration of ossification

Abalymov, A., Poelvoorde, L. V., Atkin, V., Skirtach, A. G., Konrad, M., & Parakhonskiy, B. (2020). Alkaline phosphatase delivery system based on calcium carbonate carriers for acceleration of ossification. ACS Applied Bio Materials, 3(5), 2986-2996. doi:10.1021/acsabm.0c00053.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3276345.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
3276345.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Abalymov, A., Author
Poelvoorde, L. Van, Author
Atkin, V., Author
Skirtach, A. G., Author
Konrad, M.1, Author              
Parakhonskiy, B., Author
Affiliations:
1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

Content

show
hide
Free keywords: calcium carbonate, enzyme, alkaline phosphatase, ossification, hydrogel
 Abstract: Composite bioceramic and hydrogel-based containers harboring alkaline phosphatase are generated through encapsulation of this enzyme by its immobilization into CaCO3-based bioceramic materials in combination with a hydrogel assembly technique and subsequent gelification. A refined way of synthesis and modification allows preparing the enzyme delivery system with functionalized protection layers. The particles are characterized by electron microscopy, Fourier transform infrared (FTIR) spectroscopy, and enzyme activity measurements. Loading efficiency and loading capacity are investigated depending on particle size, time of enzyme loading, and various container compositions and enzyme concentrations. Our results reveal that the size of particles influences their morphology and this, in turn, affects the activity of the encapsulated enzymes. Various functionalizations of the surfaces, including protection by the hydrogel layer, formation of hollow silver alginate, or calcium alginate encapsulation, decrease the enzymatic activity. The presence of a good therapeutic effect on osteoblastic cells coupled with a relatively high loading capacity, biocompatibility, and ease of fabrication suggests that the developed carriers are promising candidates for efficient drug delivery, especially in the field of bone reconstruction.

Details

show
hide
Language(s): eng - English
 Dates: 2020-04-012020-05-18
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acsabm.0c00053
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Applied Bio Materials
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 (5) Sequence Number: - Start / End Page: 2986 - 2996 Identifier: -