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  Photoelectric signals generated by bovine rod outer segment disk membranes attached to a lecithin bilayer

Bauer, P. J., Bamberg, E., & Fahr, A. (1984). Photoelectric signals generated by bovine rod outer segment disk membranes attached to a lecithin bilayer. Biophysical Journal, 46(1), 111-116. doi:10.1016/S0006-3495(84)84003-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-A632-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-A63D-A
Genre: Journal Article

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 Creators:
Bauer, Paul J.1, Author
Bamberg, Ernst2, Author              
Fahr, Alfred3, Author
Affiliations:
1Institute of Neurobiology, Kernforschungsanlage (KFA), 5170 Jülich, Germany, ou_persistent22              
2Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
3Department of Physics, Biophysics Group, Free University (FU) Berlin, 1000 Berlin 33, Germany, ou_persistent22              

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 Abstract: Purified bovine rod outer segment disk membranes were attached to a lecithin bilayer membrane. After photoexcitation with a 500-nm flash delivered by a dye laser, a negative photovoltage was observed on the bilayer under normal ionic strengths (100 mM KCl), which had a rise phase of 1-3 ms at 20 degrees C. The photoresponse was obviously due to bleaching of rhodopsin as it decreased for successive flashes of light. It originated most probably during the metarhodopsin-I metarhodopsin-II (meta-I-II) transition of rhodopsin because it was pH dependent at 2 degrees C but not at 20 degrees C. At 10 mM KCl, i.e., under hypotonic conditions, a positive photovoltage with slower kinetics than at high salt was observed. As the disk membranes were merely attached to the bilayer membrane, the photovoltage was apparently due to a light-induced transmembrane potential change in the disk membranes. Possible electrogenic mechanisms underlying the photosignal will be discussed.

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Language(s): eng - English
 Dates: 1984-02-271983-05-101984-07-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0006-3495(84)84003-5
PMID: 6743754
 Degree: -

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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 46 (1) Sequence Number: - Start / End Page: 111 - 116 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117