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  Reconstitution of the light-driven electrogenic ion pump halorhodopsin in black lipid membranes

Bamberg, E., Hegemann, P., & Oesterhelt, D. (1984). Reconstitution of the light-driven electrogenic ion pump halorhodopsin in black lipid membranes. Biochimica et Biophysica Acta-Biomembranes, 773(1), 53-60. doi:10.1016/0005-2736(84)90549-2.

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 Creators:
Bamberg, Ernst1, Author           
Hegemann, Peter2, Author
Oesterhelt, Dieter2, Author           
Affiliations:
1Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
2Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Free keywords: Halorhodopsin; Black lipid membrane; Photocurrent; Chloride transport Halide transport; (H. halobium)
 Abstract: Halorhodopsin-containing membrane fragments were isolated from Halobacterium halobium strain L-33, a bacteriorhodopsin-deficient mutant. When these so called Tween-washed membrane fragments are added to one side of a positively charged planar lipid bilayer the system becomes photoelectrically active. Under stationary light conditions photocurrent transients are obtained while the steady-state photocurrent is extremely low. The steady-state photocurrent, however, is considerably increased when the lipid bilayer is doped with the Cl/OH exchanging carrier triphenyltin together with a proton carrier. These results suggest that halorhodopsin is associated with the underlying black lipid membrane in a sandwich-like structure. The photoresponse occurs only in the presence of Cl, Br and I with half-saturation concentrations in the range of 1–10 mM and is virtually independent on the type of the cation (Na+, K+, Mg2+) present. With other anions, such as SO4−2, Fand NO3 no photoresponse was obtained. The results provide direct evidence that halorhodopsin is a light-driven electrogenic pump with a high specificity for halides.

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Language(s): eng - English
 Dates: 1984-01-101983-10-052002-12-161984-06-13
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2736(84)90549-2
 Degree: -

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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 773 (1) Sequence Number: - Start / End Page: 53 - 60 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702