English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Intelligent image-based deformation-assisted cell sorting with molecular specificity

Nawaz, A. A., Urbanska, M., Herbig, M., Nötzel, M., Kräter, M., Rosendahl, P., et al. (2020). Intelligent image-based deformation-assisted cell sorting with molecular specificity. Nature Methods, 17(6), 595-599. doi:10.1038/s41592-020-0831-y.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0007-A82B-C Version Permalink: http://hdl.handle.net/21.11116/0000-0007-A82C-B
Genre: Journal Article

Files

show Files
hide Files
:
s41592-020-0831-y.pdf (Publisher version), 7MB
 
File Permalink:
-
Name:
s41592-020-0831-y.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for the Science of Light, MELI; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Nawaz, Ahmad Ahsan1, 2, 3, Author              
Urbanska, Marta1, 2, 3, Author              
Herbig, Maik3, Author
Nötzel, Martin3, Author
Kräter, Martin1, 2, 3, Author              
Rosendahl, Philipp3, 4, Author
Herold, Christoph4, Author
Töpfner, Nicole3, Author
Kubánková, Markéta1, 2, Author              
Goswami, Ruchi1, 2, 3, Author              
Abuhattum Hofemeier, Shada1, 2, 3, Author              
Reichel, Felix1, 2, 3, Author              
Müller, Paul1, 2, 3, Author              
Taubenberger, Anna3, Author
Girardo, Salvatore1, 2, 3, Author              
Jacobi, Angela3, Author
Guck, Jochen1, 2, 3, Author              
Affiliations:
1Guck Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164416              
2Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              
3Technische Universität Dresden, ou_persistent22              
4Zellmech Dresden GmbH, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels. Sorting RT-FDC combines real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves to transfer molecular specificity to label-free, image-based cell sorting using an efficient deep neural network.

Details

show
hide
Language(s): eng - English
 Dates: 2020-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41592-020-0831-y
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 17 (6) Sequence Number: - Start / End Page: 595 - 599 Identifier: ISSN: 1548-7091