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  A switch in pdgfrb+ cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord

Tsata, V., Möllmert, S., Schweitzer, C., Kolb, J., Möckel, C., Böhm, B., Rosso, G., Lange, C., Lesche, M., Hammer, J., Kesavan, G., Beis, D., Guck, J., Brand, M., & Wehner, D. (2021). A switch in pdgfrb+ cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord. Developmental Cell, 56(4), 509-524. doi:10.1016/j.devcel.2020.12.009.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0007-A7F8-5 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0009-E106-2
資料種別: 学術論文

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 作成者:
Tsata, Vasiliki1, 著者
Möllmert, Stephanie2, 3, 著者           
Schweitzer, Christine2, 3, 著者           
Kolb, Julia3, 4, 著者           
Möckel, Conrad2, 3, 著者           
Böhm, Benjamin2, 3, 著者           
Rosso, Gonzalo5, 6, 著者           
Lange, Christian1, 著者
Lesche, Mathias1, 著者
Hammer, Juliane1, 著者
Kesavan, Gokul1, 著者
Beis, Dimitris1, 著者
Guck, Jochen2, 3, 7, 著者           
Brand, Michael1, 著者
Wehner, Daniel3, 4, 著者           
所属:
1external, ou_persistent22              
2Guck Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164416              
3Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              
4Wehner Research Group, Guck Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_3358768              
5Guests, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364696              
6Institute of Physiology II, University of Münster, 48149 Münster, Germany, ou_persistent22              
7Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany, ou_persistent22              

内容説明

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キーワード: spinal cord, regeneration, axon, ECM, myoseptal cells, perivascular cells, PDGFRβ, zebrafish, fibroblast, optoablation
 要旨: In mammals, perivascular cell-derived scarring after spinal cord injury impedes axonal regrowth. In contrast, the extracellular matrix (ECM) in the spinal lesion site of zebrafish is permissive and required for axon regeneration. However, the cellular mechanisms underlying this interspecies difference have not been investigated. Here, we show that an injury to the zebrafish spinal cord triggers recruitment of pdgfrb+ myoseptal and perivascular cells in a PDGFR signaling-dependent manner. Interference with pdgfrb+ cell recruitment or depletion of pdgfrb+ cells inhibits axonal regrowth and recovery of locomotor function. Transcriptional profiling and functional experiments reveal that pdgfrb+ cells upregulate expression of axon growth-promoting ECM genes (cthrc1a and col12a1a/b) and concomitantly reduce synthesis of matrix molecules that are detrimental to regeneration (lum and mfap2). Our data demonstrate that a switch in ECM composition is critical for axon regeneration after spinal cord injury and identify the cellular source and components of the growth-promoting lesion ECM.

資料詳細

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言語: eng - English
 日付: 2021-01-06
 出版の状態: オンラインで出版済み
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1016/j.devcel.2020.12.009
 学位: -

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出版物 1

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出版物名: Developmental Cell
種別: 学術雑誌
 著者・編者:
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出版社, 出版地: Cambridge, Mass. : Cell Press
ページ: - 巻号: 56 (4) 通巻号: - 開始・終了ページ: 509 - 524 識別子(ISBN, ISSN, DOIなど): ISSN: 1534-5807
CoNE: https://pure.mpg.de/cone/journals/resource/111006902714134