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  Bacterial expression of immunoglobulin fragments

Skerra, A. (1993). Bacterial expression of immunoglobulin fragments. Current Opinion in Immunology, 5(2), 256-262. doi:10.1016/0952-7915(93)90014-j.

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 Creators:
Skerra, Arne1, Author              
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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 Abstract: The expression of Ig fragments in Escherichia coli permits rapid access to engineered molecules with antigen-binding properties. While the expression in a functional state by secretion to the periplasm is the standard method for the production of Fv and Fab fragments, single chain Fv fragments are mainly produced by refolding from insoluble aggregates. Although all of these Ig fragments serve as valuable aids in the study of antigen binding, their different biochemical properties must be considered when using them as research tools or for medical applications. In addition to these simple univalent antibody fragments, the bacterial expression of bivalent and bispecific versions and of hybrid proteins with novel effector functions is gaining increasing importance.

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Language(s): eng - English
 Dates: 2003-09-121993-04
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0952-7915(93)90014-j
PMID: 8507403
 Degree: -

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Title: Current Opinion in Immunology
  Other : Curr. Opin. Immunol.
Source Genre: Journal
 Creator(s):
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Publ. Info: Philadelphia, PA, USA : Elsevier Current Trends
Pages: - Volume / Issue: 5 (2) Sequence Number: - Start / End Page: 256 - 262 Identifier: ISSN: 0952-7915
CoNE: https://pure.mpg.de/cone/journals/resource/954925575978