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  PWD/Ph-encoded genetic variants modulate the cellular Wnt/β-Catenin response to suppress ApcMin-triggered intestinal tumor formation

Farrall, A., Lienhard, M., Grimm, C., Kuhl, H., Sluka, S. H. M., Caparros Rodriguez, M., et al. (2021). PWD/Ph-encoded genetic variants modulate the cellular Wnt/β-Catenin response to suppress ApcMin-triggered intestinal tumor formation. Cancer research: an official organ of the American Association for Cancer Research, 81(1), 38-49. doi:10.1158/0008-5472.CAN-20-1480.

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© 2020 American Association for Cancer Research
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 Creators:
Farrall, Alexandra1, Author              
Lienhard, Matthias2, Author              
Grimm, Christina, Author
Kuhl, Heiner, Author
Sluka, Susanna H. M. 1, Author
Caparros Rodriguez, Marta1, Author              
Forejt, Jiri , Author
Timmermann, Bernd3, Author              
Herwig, Ralf2, Author              
Herrmann, Bernhard G.1, Author              
Morkel, Markus, Author
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              
2Bioinformatics (Ralf Herwig), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385701              
3Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479670              

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 Abstract: Genetic predisposition affects the penetrance of tumor-initiating mutations, such as APC mutations that stabilize β-catenin and cause intestinal tumors in mice and humans. However, the mechanisms involved in genetically predisposed penetrance are not well understood. Here, we analyzed tumor multiplicity and gene expression in tumor-prone ApcMin/+ mice on highly variant C57BL/6J (B6) and PWD/Ph (PWD) genetic backgrounds. (B6 × PWD) F1 APCMin offspring mice were largely free of intestinal adenoma, and several chromosome substitution (consomic) strains carrying single PWD chromosomes on the B6 genetic background displayed reduced adenoma numbers. Multiple dosage-dependent modifier loci on PWD chromosome 5 each contributed to tumor suppression. Activation of β-catenin–driven and stem cell–specific gene expression in the presence of ApcMin or following APC loss remained moderate in intestines carrying PWD chromosome 5, suggesting that PWD variants restrict adenoma initiation by controlling stem cell homeostasis. Gene expression of modifier candidates and DNA methylation on chromosome 5 were predominantly cis controlled and largely reflected parental patterns, providing a genetic basis for inheritance of tumor susceptibility. Human SNP variants of several modifier candidates were depleted in colorectal cancer genomes, suggesting that similar mechanisms may also affect the penetrance of cancer driver mutations in humans. Overall, our analysis highlights the strong impact that multiple genetic variants acting in networks can exert on tumor development.

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 Dates: 2020-10-152020-11-052021-01
 Publication Status: Published in print
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 Identifiers: DOI: 10.1158/0008-5472.CAN-20-1480
PMID: 33154092
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Title: Cancer research : an official organ of the American Association for Cancer Research
  Other : Cancer Res.
Source Genre: Journal
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Publ. Info: Baltimore, Md. : Waverly Press
Pages: - Volume / Issue: 81 (1) Sequence Number: - Start / End Page: 38 - 49 Identifier: ISSN: 0008-5472
CoNE: https://pure.mpg.de/cone/journals/resource/991042743115962