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  Metabolic Dynamics of In Vitro CD8+ T Cell Activation

Edwards-Hicks, J., Mitterer, M., Pearce, E. L., & Buescher, J. M. (2020). Metabolic Dynamics of In Vitro CD8+ T Cell Activation. Metabolites, 11. doi:10.3390/metabo11010012.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-ABCB-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-ABD3-A
Genre: Journal Article

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Edwards-Hicks et al..pdf (Publisher version), 3MB
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2020
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https://www.mdpi.com/2218-1989/11/1/12 (Publisher version)
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 Creators:
Edwards-Hicks, Joy1, Author
Mitterer, Michael1, Author
Pearce, Erika Laine1, Author              
Buescher, Joerg M.1, Author              
Affiliations:
1Department Immunometabolism, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243648              

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Free keywords: FIA-MS; LC-MS; T cell; activation; lipidomics; metabolic reprogramming; metabolomics; time course.
 Abstract: CD8+ T cells detect and kill infected or cancerous cells. When activated from their naïve state, T cells undergo a complex transition, including major metabolic reprogramming. Detailed resolution of metabolic dynamics is needed to advance the field of immunometabolism. Here, we outline methodologies that when utilized in parallel achieve broad coverage of the metabolome. Specifically, we used a combination of 2 flow injection analysis (FIA) and 3 liquid chromatography (LC) methods in combination with positive and negative mode high-resolution mass spectrometry (MS) to study the transition from naïve to effector T cells with fine-grained time resolution. Depending on the method, between 54% and 98% of measured metabolic features change in a time-dependent manner, with the major changes in both polar metabolites and lipids occurring in the first 48 h. The statistical analysis highlighted the remodeling of the polyamine biosynthesis pathway, with marked differences in the dynamics of precursors, intermediates, and cofactors. Moreover, phosphatidylcholines, the major class of membrane lipids, underwent a drastic shift in acyl chain composition with polyunsaturated species decreasing from 60% to 25% of the total pool and specifically depleting species containing a 20:4 fatty acid. We hope that this data set with a total of over 11,000 features recorded with multiple MS methodologies for 9 time points will be a useful resource for future work.

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Language(s): eng - English
 Dates: 2020-12-28
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/metabo11010012
 Degree: -

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Title: Metabolites
Source Genre: Journal
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Publ. Info: MDPI AG
Pages: - Volume / Issue: 11 Sequence Number: - Start / End Page: - Identifier: Other: 2218-1989
CoNE: https://pure.mpg.de/cone/journals/resource/2218-1989