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  Effect of surface roughness of carbon support films on high-resolution electron diffraction of two-dimensional protein crystals

Butt, H.-J., Wang, D., Hansma, P., & Kühlbrandt, W. (1991). Effect of surface roughness of carbon support films on high-resolution electron diffraction of two-dimensional protein crystals. Ultramicroscopy, 36(4), 307-318. doi:10.1016/0304-3991(91)90123-N.

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 Urheber:
Butt, Hans-Jürgen1, Autor           
Wang, D.N.2, Autor
Hansma, P.K.3, Autor
Kühlbrandt, Werner2, Autor           
Affiliations:
1Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
2European Molecular Biology Laboratory, 6900 Heidelberg, Germany, ou_persistent22              
3Department of Physics, University of California, Santa Barbara, CA 93106, USA, ou_persistent22              

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 Zusammenfassung: The surface roughness of carbon films, which are used as a specimen support in high-resolution electron microscopy, has been investigated by atomic force microscopy. Carbon films were prepared by evaporating carbon onto mica. We found that the carbon surface that had been in contact with the mica was between 3 and 9 times smoother than the surface which had faced the carbon source. Surfaces of carbon films prepared by multiple evaporation were smoother than films evaporated in a single step. The surface roughness on the mica side of these films was close to that of mica. Two-dimensional crystals of plant light-harvesting complex yielded isotropically sharp high-resolution electron diffraction patterns at high tilt angles only when supported on the smoothest carbon films, produced by multiple evaporation.

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Sprache(n): eng - English
 Datum: 1990-09-301991-03-182002-08-191991-09-01
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/0304-3991(91)90123-N
 Art des Abschluß: -

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Titel: Ultramicroscopy
  Kurztitel : Ultramicroscopy
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Amsterdam : North-Holland
Seiten: - Band / Heft: 36 (4) Artikelnummer: - Start- / Endseite: 307 - 318 Identifikator: ISSN: 0304-3991
CoNE: https://pure.mpg.de/cone/journals/resource/954925512451