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  Prebiotically Plausible RNA Activation Compatible with Ribozyme-Catalyzed Ligation

Song, E. Y., Jimenez, E. I., Lin, H., Le Vay, K., Krishnamurthy, R., & Mutschler, H. (2021). Prebiotically Plausible RNA Activation Compatible with Ribozyme-Catalyzed Ligation. Angewandte Chemie International Edition. doi:10.1002/anie.202010918.

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Genre: Journal Article
Subtitle : Communication

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© 2020 The Authors. Open access funding enabled and organized by Projekt DEAL.
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 Creators:
Song, Emilie Yeonwha1, Author              
Jimenez, Eddy Ivanhoe2, Author
Lin, Huacan2, Author
Le Vay, Kristian1, Author              
Krishnamurthy, Ramanarayanan2, Author
Mutschler, Hannes1, Author              
Affiliations:
1Mutschler, Hannes / Biomimetic Systems, Max Planck Institute of Biochemistry, Max Planck Society, ou_2466697              
2external, ou_persistent22              

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Free keywords: TEMPLATE-DIRECTED LIGATION; HAIRPIN RIBOZYME; PHOSPHORYLATION; OLIGOMERIZATION; NUCLEOBASES; HYDROLYSIS; ORIGIN; WATER; PHChemistry; diamidophosphate; early Earth; prebiotic chemistry; ribozymes; RNA;
 Abstract: RNA-catalyzed RNA ligation is widely believed to be a key reaction for primordial biology. However, since typical chemical routes towards activating RNA substrates are incompatible with ribozyme catalysis, it remains unclear how prebiotic systems generated and sustained pools of activated building blocks needed to form increasingly larger and complex RNA. Herein, we demonstrate in situ activation of RNA substrates under reaction conditions amenable to catalysis by the hairpin ribozyme. We found that diamidophosphate (DAP) and imidazole drive the formation of 2 ',3 '-cyclic phosphate RNA mono- and oligonucleotides from monophosphorylated precursors in frozen water-ice. This long-lived activation enables iterative enzymatic assembly of long RNAs. Our results provide a plausible scenario for the generation of higher-energy substrates required to fuel ribozyme-catalyzed RNA synthesis in the absence of a highly evolved metabolism.

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Language(s): eng - English
 Dates: 2020-102021
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000596882500001
DOI: 10.1002/anie.202010918
 Degree: -

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Project name : Project‐ID 364653263—TRR 235
Grant ID : 364653263
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851