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  Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ

Said, N., Hilal, T., Sunday, N. D., Khatri, A., Bürger, J., Mielke, T., et al. (2021). Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ. Science, 371(6524): eabd1673. doi:10.1126/science.abd1673.

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Said, Nelly , Author
Hilal, Tarek , Author
Sunday, Nicholas D. , Author
Khatri, Ajay , Author
Bürger, Jörg1, Author              
Mielke, Thorsten1, Author              
Belogurov, Georgiy A. , Author
Loll, Bernhard, Author
Sen, Ranjan , Author
Artsimovitch, Irina , Author
Wahl, Markus C. , Author
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1Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              

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 Abstract: Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo–electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ρ on a pathway to terminating NusA/NusG-modified elongation complexes. An open ρ ring contacts NusA, NusG, and multiple regions of RNA polymerase, trapping and locally unwinding proximal upstream DNA. NusA wedges into the ρ ring, initially sequestering RNA. Upon deflection of distal upstream DNA over the RNA polymerase zinc-binding domain, NusA rotates underneath one capping ρ subunit, which subsequently captures RNA. After detachment of NusG and clamp opening, RNA polymerase loses its grip on the RNA:DNA hybrid and is inactivated. Our structural and functional analyses suggest that ρ, and other termination factors across life, may use analogous strategies to allosterically trap transcription complexes in a moribund state.

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Language(s): eng - English
 Dates: 2020-10-262020-11-262021-01-01
 Publication Status: Published in print
 Pages: -
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 Identifiers: DOI: 10.1126/science.abd1673
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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 371 (6524) Sequence Number: eabd1673 Start / End Page: - Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1