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  Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes

Zhang, Y. W., Mess, J., & Cabezas-Wallscheid, N. (2021). Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes. Methods in Molecular Biology, 2185, 307-316. doi:10.1007/978-1-0716-0810-4_19.

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Zhang et al..pdf (Publisher version), 249KB
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2020
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Springer Science + Business Media
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 Creators:
Zhang, Yu Wei1, Author
Mess, Julian1, Author
Cabezas-Wallscheid, Nina1, Author              
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, ou_2243641              

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Free keywords: Acute myeloid leukemia; Hematopoietic stem cells; Lentiviral transduction; Leukemia stem cells; Retroviral transduction; Transplantation
 Abstract: Acute myeloid leukemia (AML) is a disease caused by multiple distinct genomic events in the hematopoietic stem cell and progenitor compartment. To gain insight into the link between genetic mutations in AML and their clinical significance, AML mouse models are often employed. However, the breeding of genetically modified mouse models is a resource-intensive and time-consuming endeavor. Here, we describe a viral-based protocol to study the role of candidate leukemia stem cell (LSC) genes. Transplantation of virally transduced oncogenic drivers for AML with virally altered expression of candidate leukemia associated genes in murine primary bone marrow cells, is an effective alternative method to assess the impact of cooperating mutations in AML.

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Language(s): eng - English
 Dates: 2020-11-102021
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-0716-0810-4_19
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
 Creator(s):
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Publ. Info: US : Springer
Pages: - Volume / Issue: 2185 Sequence Number: - Start / End Page: 307 - 316 Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544