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  Single-cell lineage tracing approaches in hematology research - technical consideration

Carrelha, J., Lin, D. S., Rodriguez-Fraticelli, A. E., Luis, T. C., Wilkinson, A. C., Cabezas-Wallscheid, N., et al. (2020). Single-cell lineage tracing approaches in hematology research - technical consideration. Experimental Hematology, 89, 26-36. doi: 10.1016/j.exphem.2020.07.007.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-AF1B-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-803E-2
Genre: Journal Article

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Carrelha.pdf (Publisher version), 991KB
 
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Carrelha.pdf
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Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
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application/pdf
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Copyright Date:
2020
Copyright Info:
ISEH - Society for Hematology and Stem Cells
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https://www.sciencedirect.com/science/article/abs/pii/S0301472X20303015?via%3Dihub (Publisher version)
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 Creators:
Carrelha, Joana1, Author
Lin, Dawn S1, Author
Rodriguez-Fraticelli, Alejo E1, Author
Luis, Tiago C1, Author
Wilkinson, Adam C1, Author
Cabezas-Wallscheid, Nina2, Author           
Tremblay, Cedric S1, Author
Haas, Simon1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, ou_2243641              

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 Abstract: The coordinated differentiation of hematopoietic stem and progenitor cells (HSPCs) into the various mature blood cell types is responsible for sustaining blood and immune system homeostasis. The cell fate decisions underlying this important biological process are made at the level of single cells. Methods to trace the fate of single cells are therefore essential for understanding hematopoietic system activity in health and disease and have had a major impact on how we understand and represent hematopoiesis. Here, we discuss the basic methodologies and technical considerations for three important clonal assays: single-cell transplantation, lentiviral barcoding, and Sleeping Beauty barcoding. This perspective is a synthesis of presentations and discussions from the 2019 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session and the 2019 ISEH Winter Webinar.

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Language(s): eng - English
 Dates: 2020-09
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.exphem.2020.07.007
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Title: Experimental Hematology
  Other : Exp. Hematol.
Source Genre: Journal
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Publ. Info: New York [etc.] : Elsevier
Pages: - Volume / Issue: 89 Sequence Number: - Start / End Page: 26 - 36 Identifier: ISSN: 0301-472X
CoNE: https://pure.mpg.de/cone/journals/resource/954925510391