Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Adult blood stem cell localization reflects the abundance of reported bone marrow niche cell types and their combinations

Kokkaliaris, K. D., Kunz, L., Cabezas-Wallscheid, N., Christodoulou, C., Renders, S., Camargo, F., et al. (2020). Adult blood stem cell localization reflects the abundance of reported bone marrow niche cell types and their combinations. Blood, 136, 2296-2307. doi:10.1182/blood.2020006574.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Kokkaliaris et al..pdf (Verlagsversion), 3MB
 
Datei-Permalink:
-
Name:
Kokkaliaris et al..pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Urheber

einblenden:
ausblenden:
 Urheber:
Kokkaliaris, Konstantinos D1, Autor
Kunz, Leo1, Autor
Cabezas-Wallscheid, Nina2, Autor           
Christodoulou, Constantina1, Autor
Renders, Simon1, Autor
Camargo, Fernando1, Autor
Trumpp, Andreas1, Autor
Scadden, David T1, Autor
Schroeder, Timm1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, ou_2243641              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: The exact localization of hematopoietic stem cells (HSCs) in their native bone marrow (BM) microenvironment remains controversial, because multiple cell types have been reported to physically associate with HSCs. In this study, we comprehensively quantified HSC localization with up to 4 simultaneous (9 total) BM components in 152 full-bone sections from different bone types and 3 HSC reporter lines. We found adult femoral α-catulin-GFP+ or Mds1GFP/+Flt3Cre HSCs proximal to sinusoids, Cxcl12 stroma, megakaryocytes, and different combinations of those populations, but not proximal to bone, adipocyte, periarteriolar, or Schwann cells. Despite microanatomical differences in femurs and sterna, their adult α-catulin-GFP+ HSCs had similar distributions. Importantly, their microenvironmental localizations were not different from those of random dots, reflecting the relative abundance of imaged BM populations rather than active enrichment. Despite their functional heterogeneity, dormant label-retaining (LR) and non-LR hematopoietic stem and progenitor cells both had indistinguishable localization from α-catulin-GFP+ HSCs. In contrast, cycling juvenile BM HSCs preferentially located close to Cxcl12 stroma and farther from sinusoids/megakaryocytes. We expect our study to help resolve existing confusion regarding the exact localization of different HSC types, their physical association with described BM populations, and their tissue-wide combinations.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2020-11-12
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1182/blood.2020006574
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Blood
  Andere : Blood
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Philadelphia, Pa. : W.B. Saunders
Seiten: - Band / Heft: 136 Artikelnummer: - Start- / Endseite: 2296 - 2307 Identifikator: ISSN: 0006-4971
CoNE: https://pure.mpg.de/cone/journals/resource/954925385125