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  Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments

Andersson, R., Safari, C., Båth, P., Bosman, R., Shilova, A., Dahl, P., et al. (2019). Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments. Acta Crystallographica Section D: Structural Biology, 75(10), 937-946. doi:10.1107/s2059798319012695.

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ActaCrystD_75_2019_937.pdf (Any fulltext), 971KB
 
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 Creators:
Andersson, Rebecka, Author
Safari, Cecilia, Author
Båth, Petra, Author
Bosman, Robert, Author
Shilova, Anastasya, Author
Dahl, Peter, Author
Ghosh, Shilova, Author
Dunge, Andreas, Author
Kjeldsen-Jensen, Rasmus, Author
Nan, Jie, Author
Shoeman, Robert L.1, Author              
Kloos, Marco1, Author              
Doak, R. Bruce1, Author              
Mueller, Uwe, Author
Neutze, Richard, Author
Brändén, Gisela, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: serial crystallography; lipidic cubic phase; membrane proteins; protein crystallization
 Abstract: Serial crystallography is having an increasing impact on structural biology. This emerging technique opens up new possibilities for studying protein structures at room temperature and investigating structural dynamics using time-resolved X-ray diffraction. A limitation of the method is the intrinsic need for large quantities of well ordered micrometre-sized crystals. Here, a method is presented to screen for conditions that produce microcrystals of membrane proteins in the lipidic cubic phase using a well-based crystallization approach. A key advantage over earlier approaches is that the progress of crystal formation can be easily monitored without interrupting the crystallization process. In addition, the protocol can be scaled up to efficiently produce large quantities of crystals for serial crystallography experiments. Using the well-based crystallization methodology, novel conditions for the growth of showers of microcrystals of three different membrane proteins have been developed. Diffraction data are also presented from the first user serial crystallography experiment performed at MAX IV Laboratory.

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Language(s): eng - English
 Dates: 2019-072019-09-112019-10-012019-10-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Acta Crystallographica Section D: Structural Biology
  Abbreviation : Acta Cryst. D
Source Genre: Journal
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Publ. Info: Chester, England : International Union of Crystallography
Pages: - Volume / Issue: 75 (10) Sequence Number: - Start / End Page: 937 - 946 Identifier: ISSN: 2059-7983
CoNE: https://pure.mpg.de/cone/journals/resource/2059-7983