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  Current status and future opportunities for serial crystallography at MAX IV Laboratory

Shilova, A., Lebrette, H., Aurelius, O., Nan, J., Welin, M., Kovacic, R., et al. (2020). Current status and future opportunities for serial crystallography at MAX IV Laboratory. Journal of Synchrotron Radiation, 27(5), 1095-1102. doi:10.1107/S1600577520008735.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-B079-A Version Permalink: http://hdl.handle.net/21.11116/0000-0007-B07A-9
Genre: Journal Article

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JSynchrotronRad_27_2020_1095.pdf (Any fulltext), 767KB
 
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 Creators:
Shilova, Anastasya, Author
Lebrette, Hugo, Author
Aurelius, Oskar, Author
Nan, Jie, Author
Welin, Martin, Author
Kovacic, Rebeka, Author
Ghosh, Swagatha, Author
Safari, Cecilia, Author
Friel, Ross J., Author
Milas, Mirko, Author
Matej, Zdenek, Author
Högbom, Martin, Author
Brändén, Gisela, Author
Kloos, Marco, Author
Shoeman, Robert L.1, Author              
Doak, Bruce1, Author              
Ursby, Thomas, Author
Håkansson, Maria, Author
Logan, Derek T., Author
Mueller, Uwe, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: serial crystallography; macromolecular crystallography; sample delivery; high-viscosity injectors
 Abstract: Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports – silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

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Language(s): eng - English
 Dates: 2020-04-132020-06-282020-08-212020-09-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S1600577520008735
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Title: Journal of Synchrotron Radiation
  Abbreviation : J. Synchrotron Radiat.
Source Genre: Journal
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Publ. Info: Wiley-Blackwell on behalf of the IUCr
Pages: - Volume / Issue: 27 (5) Sequence Number: - Start / End Page: 1095 - 1102 Identifier: ISSN: 1600-5775
ISSN: 0909-0495
CoNE: https://pure.mpg.de/cone/journals/resource/954925562624