非表示:
キーワード:
Band 3 protein; cRNA expression; Oligonucleotide-directed; mutagenesis; Inhibitor binding site; (Xenopus oocyte)
要旨:
After functional expression of mouse erythroid band 3 by cRNA microinjection into Xenopus oocytes, 36Cl− efflux is irreversibly inhibited by H2DIDS. When a cRNA is injected that is derived from a cDNA in which the nucleotides encoding for lysine-558 were replaced by nucleotides encoding for asparagine, transport and inhibition of transport by H2DIDS still occur. However, when measured under conditions where no intramolecular crosslinking takes place the inhibition by H2DIDS is no longer irreversible. This indicates that thiourea bond formation between H2DIDS and band 3 takes place at Lys-558.
