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  P2Y1 receptor mediated neuronal fibre outgrowth in organotypic brain slice co-cultures

Heine, C., Sygnecka, K., Scherf, N., Grohmann, M., Bräsigk, A., & Franke, H. (2015). P2Y1 receptor mediated neuronal fibre outgrowth in organotypic brain slice co-cultures. Neuropharmacology, 93, 252-266. doi:10.1016/j.neuropharm.2015.02.001.

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 Creators:
Heine, Claudia1, Author
Sygnecka, Katja1, Author
Scherf, Nico1, Author              
Grohmann, Marcus1, Author
Bräsigk, Annett1, Author
Franke, Heike1, Author
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1External Organizations, ou_persistent22              

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Free keywords: Purinergic stimulation; P2Y1 receptors; Organotypic slice co-culture; Dopaminergic system; Fiber growth; PI3K/Akt; ERK1/2
 Abstract: Extracellular purines have multiple functional roles in development, plastic remodelling, and regeneration of the CNS by stimulating certain P2X/Y receptor (R) subtypes. In the present study we elucidated the involvement of P2YRs in neuronal fibre outgrowth in the developing nervous system. We particularly focused on the P2Y1R subtype and the dopaminergic system, respectively. For this purpose, we used organotypic slice co-cultures consisting of the ventral tegmental area/substantia nigra (VTA/SN) and the prefrontal cortex (PFC). After detecting the presence of the P2Y1R in VTA/SN, PFC, and on outgrowing fibres in the border region (e.g. on glial processes) connecting both brain slices, we could show that pharmacological modulation of the receptor influenced neuronal fibre outgrowth. Biocytin-tracing and tyrosine hydroxylase-immunolabelling together with quantitative image analysis revealed a significant increase in fibre growth in the border region of the co-cultures after treatment with ADPβS (P2Y1,12,13R agonist). The observed stimulatory potential of ADPβS was inhibited by pre-treatment with the P2X/YR antagonist PPADS. In P2Y1R knockout (P2Y1R−/−) mice, the ADPβS-induced stimulatory effect was absent, while growth was significantly enhanced in the co-cultures of the respective wild-type. This observation was confirmed in entorhino-hippocampal co-cultures, an example of a different projection system, expressing the P2Y1R. Using wortmannin and PD98059 we further showed that PI3K/Akt and MAPK/ERK cascades are involved in the mechanism underlying ADPβS-induced fibre growth. In conclusion, the data of this study clearly indicate that activation of the P2Y1R stimulates fibre growth and thereby emphasises the general role of this particular receptor subtype during development and regeneration.

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Language(s): eng - English
 Dates: 2015-01-302014-10-082015-02-012015-02-132015-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.neuropharm.2015.02.001
PMID: 25683778
 Degree: -

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Title: Neuropharmacology
  Other : Neuropharmacol.
Source Genre: Journal
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Publ. Info: Amsterdam [etc.] : Pergamon
Pages: - Volume / Issue: 93 Sequence Number: - Start / End Page: 252 - 266 Identifier: ISSN: 0028-3908
CoNE: https://pure.mpg.de/cone/journals/resource/954925428257